Esforços consideráveis foram feitos na pesquisa da possibilidade de transplante de células-tronco/progenitoras neuronais entéricas para repovoar o intestino aganglionar; no entanto, essa terapia permanece limitada a estudos em animais.[84]Sandgren K, Ekblad E, Larsson LT. Survival of neurons and interstitial cells of Cajal after autotransplantation of myenteric ganglia from small intestine in the lethal spotted mouse. Pediatr Surg Int. 2000;16:272-6.
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[85]Hu H, Ding Y, Mu W, et al. DRG-derived neural progenitors differentiate into functional enteric neurons following transplantation in the postnatal colon. Cell Transplant. 2019 Feb;28(2):157-69.
https://www.doi.org/10.1177/0963689718811061
http://www.ncbi.nlm.nih.gov/pubmed/30442032?tool=bestpractice.com
Edição de genoma
O desenvolvimento da tecnologia CRISPR/Cas9 possibilitou avanços nessa potencial terapia para a doença de Hirschsprung; no entanto, isso limita-se a modelos animais e pesquisas laboratoriais usando linhagens de células-tronco pluripotentes induzidas por humanos.[86]Lai FP, Lau ST, Wong JK, et al. Correction of Hirschsprung-associated mutations in human induced pluripotent stem cells via clustered regularly interspaced short palindromic repeats/Cas9, restores neural crest cell function. Gastroenterology. 2017 Jul;153(1):139-53.
https://www.doi.org/10.1053/j.gastro.2017.03.014
http://www.ncbi.nlm.nih.gov/pubmed/28342760?tool=bestpractice.com
[87]Rodríguez-Rodríguez DR, Ramírez-Solís R, Garza-Elizondo MA, et al. Genome editing: A perspective on the application of CRISPR/Cas9 to study human diseases (Review). Int J Mol Med. 2019 Apr;43(4):1559-74.
https://www.doi.org/10.3892/ijmm.2019.4112
http://www.ncbi.nlm.nih.gov/pubmed/30816503?tool=bestpractice.com
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