Tests

1st tests to order

CBC and peripheral blood smear

Test
Result
Test

To look for other hematologic disorders mimicking scurvy.

Result

normocytic anemia (expected); microcytic or megaloblastic anemia if other nutritional deficiencies are present

serum ascorbic acid

Test
Result
Test

Ordered during initial evaluation to help to identify the amount and severity of vitamin C deficiency and to exclude hematologic disorders mimicking scurvy.[1][64] Sensitivity and specificity are high.

Scurvy has not been reported in patients with adequate levels of vitamin C, except in people previously on high daily doses of vitamin C with rapid decline in daily dose.[26] Alternative diagnoses should be strongly considered.[1]

Result

deficient level (high risk): <0.2 mg/100 mL; low level (medium risk): 0.2-0.29 mg/100 mL; acceptable level (low risk): >0.3 mg/100 mL

leukocyte ascorbic acid

Test
Result
Test

Ordered during initial evaluation to help to identify the amount and severity of vitamin C deficiency and to exclude hematologic disorders mimicking scurvy.[1][64] Sensitivity and specificity are high.

Scurvy has not been reported in patients with adequate levels of vitamin C, except in people previously on high daily doses of vitamin C with rapid decline in daily dose.[26] Alternative diagnoses should be strongly considered.[1]

Result

deficient level (high risk): <57 nmol/10^8 cells; low level (medium risk): 57-114 nmol/10^8 cells; acceptable level (low risk): >114 nmol/10^8 cells

whole blood ascorbic acid

Test
Result
Test

Ordered during initial evaluation to help to identify the amount and severity of vitamin C deficiency and to exclude hematologic disorders mimicking scurvy.[1][64] Sensitivity and specificity are high.

Scurvy has not been reported in patients with adequate levels of vitamin C, except in people previously on high daily doses of vitamin C with rapid decline in daily dose.[26] Alternative diagnosis should be strongly considered.[1]

Result

deficient level (high risk): <0.2 mg/100 mL (<11 micromol/L); low level (medium risk): 0.2-0.47 mg/100 mL (11-27 micromol/L); acceptable level (low risk): >0.4 mg/100 mL (>23 micromol/L)

x-ray of knee and wrist

Test
Result
Test

Ordered during initial evaluation to exclude disorders mimicking scurvy. Sensitivity and specificity are high for some signs, as described.[1][65]

Pelkan sign shows irregularities, fragmentation, and spurs at the metaphyseal margins.

The Frankel sign is a lucent line (Trummerfeld zone or scurvy line) immediately beneath a white line at the margin of the growth plate. This sign is thought to be highly specific.

Result

corner fraction sign, a ground-glass appearance, trabecular atrophy, Pelkan sign, Frankel sign

Tests to consider

MRI of knee, wrists, and long bones in pediatric cases

Test
Result
Test

Other tests are likely of greater diagnostic yield. The MRI features may be a coincidental finding when other diagnoses are being pursued, including workup for pseudoparalysis.[66][67]

Result

T1-weighted images: bone marrow unremarkable or heterogeneous high and low signal intensities; subperiosteal fluid collection with low signal intensity; T2-weighted images: bone marrow with heterogeneous high and intermediate signal intensity; periosteum may have high signal intensity

bone marrow biopsy

Test
Result
Test

Should be ordered if scurvy diagnosis is in question, or if the patient's illness is rapidly progressing during initial treatment. Given relatively rapid response to vitamin C, this invasive test may be delayed in most cases where scurvy is strongly suspected.

Result

normal or erythroid hyperplasia

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