Tests
1st tests to order
clinical diagnosis
Test
Uveitis may be idiopathic, or associated with HLA-B27-related disease or systemic inflammatory disease, viral eye disease or other localized infections, or systemic infection. Onset and duration of the ocular symptoms offer clues to the etiology. Diagnosis of underlying disease may require investigation.
In patients with a first-episode unilateral, nongranulomatous uveitis without symptoms or systemic manifestations, no further evaluation is warranted. This is also true for patients with a recent history of trauma or surgery or with clinical signs of herpes simplex virus or herpes zoster virus infection.
If the patient presents with bilateral, recurrent, or granulomatous uveitis, further evaluation is needed. CBC, ACE level (elevated in sarcoidosis), syphilis serology, and HLA-B27 help to identify a related systemic disease. Testing for rheumatoid factor and anti-cyclic citrullinated peptide antibodies should be done in the setting of suspected rheumatoid arthritis. Antinuclear antibodies (ANA) and anti-double-stranded DNA (anti-dsDNA) antibodies are important diagnostic markers for SLE. Other HLA antigens may point to the presence of specific disorders. Elevated levels of anti-neutrophil cytoplasmic antibodies point to the presence of a vasculitic condition.
Result
diagnosis is based on history, symptoms, and eye signs
Tests to consider
CBC
Test
Nonspecific marker for infectious disease.
Result
high WBC (in infection)
erythrocyte sedimentation rate (ESR)
Test
Nonspecific inflammatory marker.
CRP should be used ahead of ESR to check for inflammation in undiagnosed conditions due to increased sensitivity and specificity in the acute phase.[18]
Result
elevated (in inflammatory disease); values >80 mm/hour are always significant
CRP
Test
Nonspecific inflammatory marker.
CRP should be used ahead of ESR to check for inflammation in undiagnosed conditions due to increased sensitivity and specificity in the acute phase.[18]
Result
elevated (in inflammatory disease)
fluorescent treponemal antibody (FTA-ABS), Venereal Disease Research Laboratory (VDRL), and rapid plasma reagin (RPR)
Test
Tests help to identify syphilis infection. Ocular manifestations of syphilis involve secondary, tertiary, and neurosyphilis, and RPR and VDRL are usually positive in these patients. A positive FTA-ABS test result does not necessarily indicate active syphilis but a history of syphilis at some point, since the test remains positive for life after syphilis infection. False-positive results may occur in Lyme disease, leprosy, and diseases with high antinuclear antibody levels. False-negative results may occur in tertiary syphilis.
Result
positive (in syphilis)
serum ACE
Test
ACE is elevated in patients with sarcoidosis. Important to note if the patient is on ACE inhibitors, as the medication will lower ACE levels.
May also be elevated in hyperthyroidism, acute hepatitis, multiple myeloma, tuberculosis, and leprosy.
Result
elevated (in sarcoidosis and some other conditions associated with uveitis)
antinuclear antibodies
Test
A general marker of collagen vascular disease.
Seen in juvenile idiopathic arthritis, and so may be particularly useful in children.
Antinuclear antibody is positive in virtually all patients with SLE.[19]
Result
positive (in many collagen vascular diseases)
HLA-B27
Test
Approximately 49% of patients with anterior uveitis test positive for HLA-B27.[10]
Presence suggests seronegative spondyloarthropathy. Often positive in recurrent iritis.
Result
positive (in many patients with uveitis)
Lyme titer
Test
A Lyme titer testing for Borrelia burgdorferi should be ordered in endemic areas.[20]
Result
positive (in Lyme disease)
purified protein derivative (PPD) skin test
Test
Should be ordered for patients with suspected tuberculosis (TB), and for those with HIV or history of alcohol abuse. Should also be ordered in endemic regions or after recent contact with an infected person.
Active TB should be ruled out if test is positive.
Special considerations should be taken when interpreting the test in patients receiving the Bacillus Calmette-Guérin vaccine (false positive) and in immunosuppressed patients (false negative).
Interferon-gamma release assays are a newer test that may help to diagnose TB.
Result
positive (in TB) if >5 mm in HIV patients, 10 mm or greater in high-risk patients, 15 mm or greater in low-risk patients
cytoplasmic anti-neutrophil cytoplasmic antibodies (c-ANCA)
Test
Most patients with granulomatosis with polyangiitis (formerly known as Wegener granulomatosis) have a positive c-ANCA.
Proteinase 3 is the c-ANCA target antigen.
Result
positive (in granulomatosis with polyangiitis)
perinuclear anti-neutrophil cytoplasmic antibodies (p-ANCA)
Test
Levels elevated in microscopic polyangiitis, Churg-Strauss syndrome, and other vasculitides.
Myeloperoxidase is the p-ANCA target antigen.
Result
elevated (in various vasculitic conditions)
anti-double-stranded DNA antibody (anti-dsDNA)
Test
Positive in systemic lupus erythematosus (SLE).
Result
positive (in SLE)
rheumatoid factor
Test
Levels elevated in rheumatoid arthritis; levels also elevated in Sjogren syndrome and other autoimmune diseases.
Result
elevated (in rheumatoid arthritis and some other autoimmune diseases)
anti-cyclic citrullinated peptide (anti-CCP) antibodies
Test
Elevated levels specific for rheumatoid arthritis; not used in children.
Result
elevated (in rheumatoid arthritis)
Bartonella henselae titer
Test
Positive serology is highly indicative of cat-scratch disease.
Result
positive (in cat-scratch fever)
toxoplasma serologic titer
Test
If positive, highly indicative of toxoplasmosis as the causative agent.
Result
positive (in toxoplasma infection)
other HLA antigens
Test
Presence may indicate the presence of specific conditions (HLA-A29 in birdshot retinochoroidopathy [a bilateral inflammatory disease affecting the choroid layer]; HLA B*0501 in Behçet disease; HLA DR*0405 in Vogt-Koyanagi-Harada syndrome, a multisystem autoimmune disorder characterized by chronic uveitis with skin, neurologic, and auditory manifestations).
Result
present or absent
biochemistry screen
Test
May identify renal and hepatic dysfunction associated with uveitis-related inflammatory diseases.
Result
may be abnormal in systemic inflammatory disorders
CXR
Test
Should be ordered to look for tuberculosis (TB) or sarcoidosis.
Result
sarcoidosis: enlarged lymph nodes or pulmonary infiltration; active TB: clear or infiltrate or consolidation, cavitary lesion with or without surrounding calcification, nodule with poorly defined margins, pleural effusion, hilar or mediastinal lymphadenopathy
polymerase chain reaction (PCR)
Test
Performed on aqueous fluid from the anterior chamber of the eye in cases of suspected viral infection.
Result
may be positive for herpes simplex virus, varicella zoster virus, or cytomegalovirus DNA
Emerging tests
vitreous biopsy
Test
Removal of vitreous fluid for analysis allows helpful diagnostic data for uveitis of unknown cause.[21] Cytologic analysis, flow cytometry, and culture may be performed.
Result
may show positive culture or cellular abnormality
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