The Framingham Offspring Study found up to 39% of US adults at risk for vitamin B12 deficiency (defined as serum vitamin B12 <350 picograms/mL).[10]Tucker KL, Rich S, Rosenberg I, et al. Plasma vitamin B-12 concentrations relate to intake source in the Framingham Offspring study. Am J Clin Nutr. 2000 Feb;71(2):514-22.
http://www.ncbi.nlm.nih.gov/pubmed/10648266?tool=bestpractice.com
Prevalence increases with advancing age, and ranges from 5% to 35% in older people depending on the population studied and the methods of diagnosis.[11]Sukumar N, Saravanan P. Investigating vitamin B12 deficiency. BMJ. 2019 May 10;365:l1865.
http://www.ncbi.nlm.nih.gov/pubmed/31076395?tool=bestpractice.com
[12]Pennypacker LC, Allen RH, Kelly JP, et al. High prevalence of cobalamin deficiency in elderly outpatients. J Am Geriatr Soc. 1992 Dec;40(12):1197-204.
http://www.ncbi.nlm.nih.gov/pubmed/1447433?tool=bestpractice.com
[13]Clarke R, Grimley Evans J, Schneede J, et al. Vitamin B12 and folate deficiency in later life. Age Ageing. 2004 Jan;33(1):34-41.
http://ageing.oxfordjournals.org/cgi/reprint/33/1/34
http://www.ncbi.nlm.nih.gov/pubmed/14695861?tool=bestpractice.com
[14]Andres E, Loukili NH, Noel E, et al. Vitamin B12 (cobalamin) deficiency in elderly patients. CMAJ. 2004 Aug 3;171(3):251-9.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC490077
http://www.ncbi.nlm.nih.gov/pubmed/15289425?tool=bestpractice.com
[15]Pfisterer KJ, Sharratt MT, Heckman GG, et al. Vitamin B12 status in older adults living in Ontario long-term care homes: prevalence and incidence of deficiency with supplementation as a protective factor. Appl Physiol Nutr Metab. 2016 Feb;41(2):219-22.
http://www.ncbi.nlm.nih.gov/pubmed/26781161?tool=bestpractice.com
[16]Wong CW, Ip CY, Leung CP, et al. Vitamin B12 deficiency in the institutionalized elderly: A regional study. Exp Gerontol. 2015 Sep;69:221-5.
http://www.ncbi.nlm.nih.gov/pubmed/26122132?tool=bestpractice.com
[17]Mineva EM, Sternberg MR, Bailey RL, et al. Fewer US adults had low or transitional vitamin B12 status based on the novel combined indicator of vitamin B12 status compared with individual, conventional markers, NHANES 1999-2004. Am J Clin Nutr. 2021 Sep 1;114(3):1070-79.
https://www.sciencedirect.com/science/article/pii/S0002916522004300?via%3Dihub
http://www.ncbi.nlm.nih.gov/pubmed/33963731?tool=bestpractice.com
In the US and the UK, the prevalence of vitamin B12 deficiency is approximately 6% in persons younger than 60 years, and nearly 20% in those older than 60 years.[18]Hunt A, Harrington D, Robinson S. Vitamin B12 deficiency. BMJ. 2014 Sep 4;349:g5226.
http://www.ncbi.nlm.nih.gov/pubmed/25189324?tool=bestpractice.com
Marginal vitamin B12 levels are found in approximately 20% of people in the US and UK.[19]Allen LH. How common is vitamin B-12 deficiency? Am J Clin Nutr. 2009 Feb;89(2):693S-6S.
https://academic.oup.com/ajcn/article/89/2/693S/4596795
http://www.ncbi.nlm.nih.gov/pubmed/19116323?tool=bestpractice.com
Studies in other world regions including Scandinavia and the Middle East have found evidence of low serum vitamin B12 (<200 picograms/mL) in 25% to 70% of the studied population.[20]Björkegren K, Svärdsudd K. Serum cobalamin, folate, methylmalonic acid and total homocysteine as vitamin B12 and folate tissue deficiency markers amongst elderly Swedes - a population-based study. J Intern Med. 2001 May;249(5):423-32.
https://onlinelibrary.wiley.com/doi/full/10.1046/j.1365-2796.2001.00819.x
http://www.ncbi.nlm.nih.gov/pubmed/11350566?tool=bestpractice.com
[21]Yajnik CS, Deshpande SS, Lubree HG, et al. Vitamin B12 deficiency and hyperhomocysteinemia in rural and urban Indians. J Assoc Physicians India. 2006 Oct;54:775-82.
http://www.ncbi.nlm.nih.gov/pubmed/17214273?tool=bestpractice.com
[22]Fakhrzadeh H, Ghotbi S, Pourebrahim R, et al. Total plasma homocysteine, folate, and vitamin B12 status in healthy Iranian adults: the Tehran homocysteine survey (2003-2004)/a cross-sectional population based study. BMC Public Health. 2006 Feb 13;6:29.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1403764
http://www.ncbi.nlm.nih.gov/pubmed/16472406?tool=bestpractice.com
[23]Loikas S, Koskinen P, Irjala K, et al. Vitamin B12 deficiency in the aged: a population-based study. Age Ageing. 2007 Mar;36(2):177-83.
http://ageing.oxfordjournals.org/cgi/content/full/36/2/177
http://www.ncbi.nlm.nih.gov/pubmed/17189285?tool=bestpractice.com
[24]Madhari RS, Boddula S, Ravindranadh P, et al. High dietary micronutrient inadequacy in peri-urban school children from a district in South India: Potential for staple food fortification and nutrient supplementation. Matern Child Nutr. 2020 Dec;16 Suppl 3:e13065.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7752122
http://www.ncbi.nlm.nih.gov/pubmed/33347729?tool=bestpractice.com
The prevalence of vitamin B12 deficiency is expected to rise with the increasing popularity of veganism and vegetarianism. The exact prevalence of deficiency among vegans and strict vegetarians is difficult to estimate due to study method heterogeneity; however, it may range from as low as 11% to as high as 90% depending on age.[25]Pawlak R, Parrott SJ, Raj S, et al. How prevalent is vitamin B(12) deficiency among vegetarians? Nutr Rev. 2013 Feb;71(2):110-7.
https://academic.oup.com/nutritionreviews/article/71/2/110/1940320?login=false
http://www.ncbi.nlm.nih.gov/pubmed/23356638?tool=bestpractice.com
[26]Herrmann W, Schorr H, Obeid R, et al. Vitamin B-12 status, particularly holotranscobalamin II and methylmalonic acid concentrations, and hyperhomocysteinemia in vegetarians. Am J Clin Nutr. 2003 Jul;78(1):131-6.
http://www.ncbi.nlm.nih.gov/pubmed/12816782?tool=bestpractice.com
Rising numbers of gastric bypass procedures have led to an increase in vitamin B12 deficiency. Roux-en-Y gastric bypass surgery may cause deficiency in up to half of patients in the initial postoperative years due to inadequate absorption.[27]Weng TC, Chang CH, Dong YH, et al. Anaemia and related nutrient deficiencies after Roux-en-Y gastric bypass surgery: a systematic review and meta-analysis. BMJ Open. 2015 Jul 16;5(7):e006964.
http://bmjopen.bmj.com/content/5/7/e006964.long
http://www.ncbi.nlm.nih.gov/pubmed/26185175?tool=bestpractice.com
[28]Schijns W, Homan J, van der Meer L, et al. Efficacy of oral compared with intramuscular vitamin B-12 supplementation after Roux-en-Y gastric bypass: a randomized controlled trial. Am J Clin Nutr. 2018 Jul 1;108(1):6-12.
https://www.sciencedirect.com/science/article/pii/S0002916522029057?via%3Dihub
http://www.ncbi.nlm.nih.gov/pubmed/29931179?tool=bestpractice.com
Vitamin B12 deficiency may be seen in 20% to 30% of women during pregnancy, and is particularly common if the woman is vegetarian or vegan.[11]Sukumar N, Saravanan P. Investigating vitamin B12 deficiency. BMJ. 2019 May 10;365:l1865.
http://www.ncbi.nlm.nih.gov/pubmed/31076395?tool=bestpractice.com
[29]Rashid S, Meier V, Patrick H. Review of vitamin B12 deficiency in pregnancy: a diagnosis not to miss as veganism and vegetarianism become more prevalent. Eur J Haematol. 2021 Apr;106(4):450-5.
https://onlinelibrary.wiley.com/doi/10.1111/ejh.13571
http://www.ncbi.nlm.nih.gov/pubmed/33341967?tool=bestpractice.com
One systematic review found that levels of vitamin B12 decrease from the first to the third trimester.[30]Sukumar N, Rafnsson SB, Kandala NB, et al. Prevalence of vitamin B-12 insufficiency during pregnancy and its effect on offspring birth weight: a systematic review and meta-analysis. Am J Clin Nutr. 2016 May;103(5):1232-51.
http://ajcn.nutrition.org/content/103/5/1232.long
http://www.ncbi.nlm.nih.gov/pubmed/27076577?tool=bestpractice.com