Tests

1st tests to order

serum sodium concentration

Test
Result
Test

Should be ordered in all patients with suspected hyponatremia.

A serum sodium concentration <135 mEq/L (corrected for hyperglycemia) confirms the presence of hyponatremia.[2][3]​​

Result

<135 mEq/L

serum electrolytes, BUN, creatinine, and glucose

Test
Result
Test

Should be ordered in all patients with suspected hyponatremia.

May reveal other electrolyte abnormalities or renal impairment.

Serum glucose (random or fasting) should be checked to exclude hyperglycemia-associated hyponatremia. If the patient is hyperglycemic, a sodium correction formula should be used. If the glucose level is >100 mg/dL, the most accurate correction formula is: corrected serum sodium (mEq/L) = measured serum sodium (mEq/L) + 2.4 x {[serum glucose (mg/dL) - 100]/100}. This formula should be used to determine if true hyponatremia is present.[42]

Result

variable

serum osmolality

Test
Result
Test

Serum osmolality can be used to differentiate between hypotonic and hypertonic hyponatremia.[2][3] [ Osmolality Estimator (serum) Opens in new window ] ​​​​​​

Serum osmolality <275 mOsm/kg: indicates hypotonic hyponatremia.

Serum osmolality >295 mOsm/kg: indicates hypertonic hyponatremia.

Serum osmolality normal: indicates isotonic hyponatremia (pseudohyponatremia).

Result

variable

urine sodium concentration

Test
Result
Test

Ordered to confirm the presence of hypovolemia or euvolemia.

A spot urine test allows urinary sodium concentration to be measured quickly and conveniently.

Urine sodium concentration, in combination with the volume status of the patient from exam, can provide further clues to the classification and etiology.

In hypovolemic hyponatremia, urine sodium concentration >20 mEq/L indicates renal sodium loss (e.g., diuretics), and ≤20 mEq/L indicates nonrenal sodium loss (e.g., gastrointestinal losses).

In hypervolemic hyponatremia, urine sodium concentration >20 mEq/L indicates acute kidney injury/chronic kidney disease or diuretic use, and ≤20 mEq/L indicates congestive heart failure, cirrhosis, or nephrotic syndrome.

In euvolemic hyponatremia, urine sodium concentration is >20 mEq/L in most patients; however, patients with a concomitant low sodium intake may have a low urinary sodium.

Result

variable

urine osmolality

Test
Result
Test

Used to further evaluate the cause of euvolemic hyponatremia.

High (≥300 mOsm/kg): indicates syndrome of inappropriate antidiuretic hormone (SIADH) due to the inappropriate dilution of plasma as a result of pathologic vasopressin release, or may also be due to drug-related effects.[2][5]​​

Intermediate (150-300 mOsm/kg): indicates potomania or a partial effect of medications or mild SIADH in conjunction with high fluid intake.[5]

Low (<100-150 mOsm/kg): indicates primary polydipsia.[5]

Note: in hyponatremia due to prolonged physical exercise with high fluid intake urine osmolality is high initially reflecting vasopressin release. This is followed by low urine osmolality as self-correction and aquaresis (loss of water) occur.

Result

variable

urine electrolytes

Test
Result
Test

Should be ordered in all patients with hyponatremia to determine electrolyte-free water excretion.

Result

variable

urine flow rate

Test
Result
Test

Volume of urine excreted in a specific time period.

Should be ordered in all patients with hyponatremia to determine electrolyte-free water excretion.

Result

variable

electrolyte-free water excretion

Test
Result
Test

Should be calculated in patients with hyponatremia using the following formula: electrolyte-free water excretion = V x [1 - (UNa + UK)/(PNa)], where V is the urine flow rate, UNa is the urine concentration of sodium (mEq/L), UK is the urine concentration of potassium (mEq/L), and PNa is the plasma concentration of sodium (mEq/L).

Resulting value indicates how much electrolyte-free water is being lost through the urine at any given time (per day). Can be used to help determine if water is being retained (negative value) as in syndrome of inappropriate antidiuretic hormone secretion (SIADH), or excreted (positive value) as in polydipsia or potomania.[43][44]

Helps to determine the treatment plan by determining ongoing water gains or losses.

Result

negative (e.g., SIADH); positive (e.g., polydipsia, potomania)

fractional excretion of sodium

Test
Result
Test

Although a spot urine sodium test can be helpful if the result is very low, the fractional excretion of sodium provides a more accurate assessment of volume status as it corrects for the effect of variations in urine volume on the urine sodium. [ Fractional Excretion of Sodium Opens in new window ]

Calculated using the following formula: [(urinary sodium concentration x plasma creatinine concentration)/(plasma sodium concentration x urinary creatinine concentration)] x 100%.

A value of <1% usually indicates pre-renal causes of hyponatremia.

Result

variable

thyroid-stimulating hormone

Test
Result
Test

Order in patients with euvolemic hyponatremia. Other thyroid function tests may also be needed to determine whether primary or secondary hypothyroidism is present.

Hypothyroidism must be excluded for a diagnosis of syndrome of inappropriate antidiuretic hormone to be made.

Result

may exclude thyroid dysfunction

serum cortisol level and/or adrenocorticotropic hormone test

Test
Result
Test

Order in patients with euvolemic hyponatremia.

Adrenal insufficiency must be excluded for a diagnosis of syndrome of inappropriate antidiuretic hormone to be made.

Result

may exclude adrenal insufficiency

serum lipids and serum protein electrophoresis

Test
Result
Test

Normal in true hyponatremia. Elevated lipids or proteins can cause pseudohyponatremia.

Most common cause of high serum proteins is multiple myeloma.

Result

may exclude pseudohyponatremia

Tests to consider

CT brain, chest, abdomen/pelvis

Test
Result
Test

Ordered to identify potential causes of syndrome of inappropriate antidiuretic hormone (SIADH; e.g., malignancy such as small cell lung cancer, gastrointestinal tract cancers; central nervous system disorders such as subarachnoid hemorrhage; or other pulmonary disease such as pneumonia) and should be guided by history and physical exam.[4][31]

At times, SIADH may be the presenting finding of a malignancy, or it may precede the malignancy diagnosis.

Result

may reveal possible causes of SIADH

other tests targeted at evaluating the underlying cause

Test
Result
Test

Other tests may be required, as determined by the history and exam, to evaluate the underlying cause, including CBC, LFTs, abdominal ultrasound, chest x-ray, ECG, echocardiogram, renal/liver biopsy, serum albumin, urinalysis, and GFR.

Result

variable (depending on cause)

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