Tests

Tests to consider

fasting blood glucose

Test
Result
Test

To screen for type 2 diabetes mellitus.

The American Diabetes Association recommends that children >10 years of age (or at onset of puberty, if it begins at an earlier age) with a body mass index (BMI) >85th percentile plus one or more other risk factors (e.g., family history of type 2 diabetes in a first- or second-degree relative, nonwhite race, and/or conditions associated with insulin resistance such as acanthosis nigricans, polycystic ovary syndrome, hypertension, dyslipidemia, small for gestational age, or maternal history of diabetes or gestational diabetes mellitus during the child’s gestation) should have a fasting blood glucose test, a 2 hour plasma glucose during a 75 g oral glucose tolerance test, or hemoglobin A1c. Screening should be conducted at a minimum of every 3 years or more frequently if BMI is increasing, or risk factor profile is deteriorating.[73]​​

Result

normal, or impaired glucose tolerance (100-125 mg/dL), or diabetic (≥126 mg/dL)

serum lipids

Test
Result
Test

To screen for dyslipidemia.

All children should be screened for lipid abnormalities with a nonfasting, non-HDL cholesterol level between ages 9 to 11 years and 17 to 21 years.[70]

A fasting lipid panel should be obtained in children with a BMI ≥85th percentile.[18]

Lipid abnormalities in children often persist into adulthood.[70]

Cutoff values are as follows.[70] Normal: cholesterol <170 mg/dL, LDL <110 mg/dL; borderline: cholesterol 170 to 199 mg/dL, LDL 110 to 129 mg/dL; elevated: cholesterol >200 mg/dL, LDL >130 mg/dL.

Result

normal or elevated

liver function tests

Test
Result
Test

To screen for nonalcoholic fatty liver disease, as most patients will be asymptomatic.

Children >10 years of age with a BMI ≥95th percentile, or with a BMI ≥85th percentile and other risk factors, should be screened twice a year with a serum alanine aminotransferase (ALT).[71]

Children should be referred to a hepatologist if ALT or AST is ≥2 times the upper limit of normal.[64]

Result

normal or elevated transaminases

Emerging tests

DEXA

Test
Result
Test

Can be used to assess total body fat.

Use of this method is limited by the expense of the method and the inability to distinguish between subcutaneous and visceral fat.

It is used mainly in the research setting.

Result

elevated for age and sex

bioelectric impedance analysis

Test
Result
Test

A noninvasive and relatively inexpensive assessment of body composition. However, measurements are highly variable, as they are affected by the patient's hydration status.[74]

Result

elevated for age and sex

abdominal CT or MRI

Test
Result
Test

Can be used to accurately measure visceral fat.[75] However, these methods are costly, and should only be done in the research setting.

Result

increased visceral fat

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