Investigations

1st investigations to order

ECG

Test
Result
Test

Perform a 12-lead ECG in all patients to rule out other causes of a patient’s symptoms.[1][2][4]

Result

normal sinus rhythm

FBC

Test
Result
Test

Order FBC to exclude anaemia as a potential cause of orthostatic tachycardia.[1]​​[4]​ See Assessment of anaemia.

Result

normal

thyroid function tests

Test
Result
Test

Order thyroid function tests (thyroid-stimulating hormone, free thyroxine, free triiodothyronine) to exclude hyperthyroidism as a differential diagnosis.[1]​​[4]​ See Graves' disease.

Result

normal

electrolytes

Test
Result
Test

Order electrolytes to rule out other causes of a patient’s presentation, such as adrenal insufficiency.[1]​​[4]​ In practice, electrolytes should also be checked after starting certain pharmacological treatments for POTS, such as fludrocortisone or desmopressin.

Result

normal

morning serum cortisol

Test
Result
Test

Order morning serum cortisol level to exclude primary adrenal insufficiency (Addison's disease) as a differential diagnosis.​​​[4][44]​​​ See Primary adrenal insufficiency.

Result

normal

Investigations to consider

Holter monitor

Test
Result
Test

Consider a 24-hour Holter monitor, which can help confirm the diagnosis by demonstrating the association between tachycardia and orthostatic changes.[2]​ A Holter monitor can also rule out supraventricular arrhythmias that may have a similar presentation to POTS.[2]

Result

tachycardia induced by orthostatic changes; no supraventricular arrhythmias[2]

echocardiogram

Test
Result
Test

Organise an echocardiogram to exclude heart failure as a differential if you suspect this from the history or there are signs of ventricular dysfunction found on examination (e.g., pitting oedema of the lower extremities, distended jugular veins).[2][4]

Result

normal

tilt-table test

Test
Result
Test

Organise a tilt-table test if:

  • The diagnosis is unclear after the initial assessment of orthostatic blood pressure and heart rate and you have a high suspicion of POTS.[2]​ In this scenario, a tilt-table test is helpful because it will provide an assessment of vital signs over a greater time period compared with a simple 10-minute standing test.​​​

    OR

  • The patient is not able to perform a 10-minute standing test.[1]​​​

Result

orthostatic tachycardia with changing position

supine and upright plasma adrenaline and noradrenaline levels

Test
Result
Test

If the patient’s symptoms do not resolve or significantly improve, consider referral to a centre experienced with the autonomic testing of POTS, where available, to organise further investigation of the underlying pathology.[2]​ This includes supine and upright plasma adrenaline and noradrenaline levels, which should be considered if hyperadrenergic POTS is suspected.[43]​ However, this should not be performed routinely because the significance for patient management and outcome is unclear.[2]

Result

plasma noradrenaline levels ≥3547 pmol/L (≥600 pg/mL);[43]​ supine adrenaline ≥213 pmol/L (≥39 pg/mL);[45]​ upright adrenaline ≥409 pmol/L (≥75 pg/mL)[45]

thermoregulatory sweat test

Test
Result
Test

If the patient’s symptoms do not resolve or significantly improve, consider referral to a centre experienced with the autonomic testing of POTS, where available, to organise further investigation of the underlying pathology.[2]​ This includes a thermoregulatory sweat test, which should be considered to detect the autonomic neuropathy associated with POTS.[2]​ However, this should not be performed routinely because the significance for patient management and outcome is unclear.[2]

Result

abnormal patterns of body sweating

quantitative sudomotor axon reflex test

Test
Result
Test

If the patient’s symptoms do not resolve or significantly improve, consider referral to a centre experienced with the autonomic testing of POTS, where available, to organise further investigation of the underlying pathology.[2]​ This includes a quantitative sudomotor axon reflex test if neuropathic POTS is suspected.[42]​ Neuropathic POTS is associated with peripheral venous pooling and reduced effective intravascular volume, which is caused by peripheral sympathetic denervation.[1][2]​​​​​ However, quantitative sudomotor axon reflex test should not be performed routinely because the significance for patient management and outcome is unclear.[2]

Result

abnormal post-ganglionic sympathetic sudomotor function[42]

valsalva manoeuvre

Test
Result
Test

If the patient’s symptoms do not resolve or significantly improve, consider referral to a centre experienced with the autonomic testing of POTS,where available, to organise further investigation of the underlying pathology.[2]​ This includes the Valsalva manoeuvre with haemodynamic monitoring, which can detect autonomic dysfunction.[2][4]​ However, this should not be performed routinely because the significance for patient management and outcome is unclear.[2]

Result

excessive increase in blood pressure at the end of the manoeuvre if the patient has hyperadrenergic POTS[4]

deep breathing test

Test
Result
Test

If the patient’s symptoms do not resolve or significantly improve, consider referral to a centre experienced with the autonomic testing of POTS, where available, to organise further investigation of the underlying pathology.[2]​ This includes the deep breathing test with haemodynamic monitoring, which can detect autonomic dysfunction.​​[4]​ However, this should not be performed routinely because the significance for patient management and outcome is unclear.[2]

Result

preserved vagal function is often found in POTS, demonstrated by the sinus arrhythmia ratio in response to deep breathing[4]

exercise testing

Test
Result
Test

Formal cardiopulmonary exercise testing can be useful as a measure of exercise capacity, which is often reduced in patients with POTS.

Result

exercise capacity baseline; often reduced in patients with POTS

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