Differentials

Zika virus infection

SIGNS / SYMPTOMS
INVESTIGATIONS
SIGNS / SYMPTOMS

Residence in/travel from a Zika-affected region in the previous 2 weeks, or unprotected sexual contact with infected individual.

Conjunctivitis (usually non-purulent) or conjunctival hyperaemia.

No signs of bleeding or haemorrhage.

Mild cases may be difficult to distinguish from dengue fever without specific diagnostic testing.

It is imperative to differentiate dengue fever from Zika virus infection in pregnancy as the latter is known to be associated with microcephaly in newborns.[83]

INVESTIGATIONS

Reverse transcriptase-polymerase chain reaction (RT-PCR) for Zika: positive.

Serology for Zika: positive (note: risk of cross-reaction with dengue antibodies).

The US Centers for Disease Control and Prevention (CDC) recommends molecular and/or serological testing for dengue and Zika viruses in patients who are at risk of infection with both viruses.[90]

The World Health Organization (WHO) has produced a tool to help physicians differentiate between dengue, chikungunya, and Zika virus infection.[85]

Chikungunya virus infection

SIGNS / SYMPTOMS
INVESTIGATIONS
SIGNS / SYMPTOMS

Peripheral arthralgia and/or arthritis occurs in almost all patients. Pain is usually severe and most commonly affects the distal joints (e.g., wrists, ankles, hands, feet). It is usually symmetric. May be prominent signs of joint inflammation (swelling, warmth, rubor).

Dermatological manifestations including hyperpigmentation of the skin (predominantly on the face), desquamation of the skin (especially the palms of hands and soles of feet), well-demarcated ulcers in the intertrigo/perigenital/perianal regions, and oral aphthous ulcers are common.

Absence of bleeding tendency and circulatory failure.[91][92]

May be difficult to distinguish from dengue fever without specific diagnostic testing.

INVESTIGATIONS

Serology for chikungunya: positive.

The WHO has produced a tool to help physicians differentiate between dengue, chikungunya, and Zika virus infection.[85]

Rickettsial diseases

SIGNS / SYMPTOMS
INVESTIGATIONS
SIGNS / SYMPTOMS

History of tick, flea, or mite bite.

Discrete maculopapular and erythematous rash, which mainly involves the soles of the feet, palms of the hands, and limbs in Rocky Mountain spotted fever, and the trunk in other Rickettsial infections.

Characteristic eschar is seen.[93]

Arthritis/arthralgia of large joints may occur.

INVESTIGATIONS

FBC: WCC and platelet count usually normal; however, thrombocytopenia can occur in severe disease.

Serology (indirect immunofluorescent antibody) for Rickettsial infections: positive.

Leptospirosis

SIGNS / SYMPTOMS
INVESTIGATIONS
SIGNS / SYMPTOMS

History of working or bathing in muddy or stagnant water or handling animals.

Conjunctival injection.[94]

INVESTIGATIONS

FBC: leukocytosis.

Urinalysis: haematuria.

Serum bilirubin: elevated.

Urea: elevated.

Serology (microscopic agglutination test) for leptospirosis: positive.

Polymerase chain reaction (PCR) assay for leptospirosis: positive.

Measles infection

SIGNS / SYMPTOMS
INVESTIGATIONS
SIGNS / SYMPTOMS

Koplik spots (i.e., red spots often with a bluish-white central dot on erythematous buccal mucosa).

Upper respiratory tract symptoms (e.g., cough, coryza) more common.

Conjunctivitis.

Maculopapular rash, predominantly on the face and ears.

INVESTIGATIONS

Serology (measles-specific IgM and IgG enzyme-linked immunosorbent assay [ELISA]) for measles: positive.

Rubella

SIGNS / SYMPTOMS
INVESTIGATIONS
SIGNS / SYMPTOMS

Maculopapular rash, which usually begins on the face and spreads from the head to the feet.

Lymphadenopathy (often posterior cervical).

Arthralgia.

Conjunctivitis.

INVESTIGATIONS

Serology (antirubella IgM or IgG): positive.

Viral culture or reverse transcription-polymerase chain reaction (RT-PCR): may be positive.

Infectious mononucleosis

SIGNS / SYMPTOMS
INVESTIGATIONS
SIGNS / SYMPTOMS

Long-standing fever (i.e., 1-2 weeks, rarely up to 5 weeks).

Cervical or generalised lymphadenopathy.

Pharyngitis.

Variable skin rash that worsens with use of beta-lactam antibiotics.

INVESTIGATIONS

FBC: lymphocytosis or atypical lymphocytosis.

Epstein-Barr virus (EBV)-specific antibodies: positive.

Meningococcal disease

SIGNS / SYMPTOMS
INVESTIGATIONS
SIGNS / SYMPTOMS

Neck pain and/or stiffness.

Photophobia.

Altered consciousness.

Seizures.

Haemorrhagic rash.

INVESTIGATIONS

Blood culture: positive forNeisseria meningitides.

Typhoid infection

SIGNS / SYMPTOMS
INVESTIGATIONS
SIGNS / SYMPTOMS

Travel history to endemic area.

Fever sometimes occurs in a stepwise fashion.

Constipation.

Rose spots (blanching erythematous maculopapular lesions) are rare; usually seen on abdomen.

Relative bradycardia (although this is neither sensitive or specific for typhoid).

Splenomegaly.

INVESTIGATIONS

Blood/urine/stool culture: positive forSalmonella enterica (S typhi serotype).

Serology (Widal test) for typhoid: positive.

Hantavirus infection

SIGNS / SYMPTOMS
INVESTIGATIONS
SIGNS / SYMPTOMS

Exposure to rodent excreta.

Dyspnoea or severe respiratory distress.

INVESTIGATIONS

Serology (IgM and IgG for Sin Nombre virus) for hantavirus: positive.

Yellow fever

SIGNS / SYMPTOMS
INVESTIGATIONS
SIGNS / SYMPTOMS

Conjunctival injection.

Jaundice.

Relative bradycardia.

INVESTIGATIONS

Serology (IgM ELISA or haemagglutination inhibition): positive, increasing IgG titres.

West Nile virus

SIGNS / SYMPTOMS
INVESTIGATIONS
SIGNS / SYMPTOMS

Lymphadenopathy.

Conjunctival injection.

Encephalitis.

INVESTIGATIONS

Serology (West Nile virus-specific IgM ELISA) or specific viral detection for West Nile virus: positive in CSF and serum.

Malaria

SIGNS / SYMPTOMS
INVESTIGATIONS
SIGNS / SYMPTOMS

Travel history to endemic area.

Characteristic paroxysms of chills and rigors followed by fever and sweats may be described.

Absence of rash.

INVESTIGATIONS

Giemsa-stained thick and thin blood smears: positive for the malaria parasite.

Rapid diagnostic tests: detection of parasite antigen or enzymes.

Crimean-Congo haemorrhagic fever

SIGNS / SYMPTOMS
INVESTIGATIONS
SIGNS / SYMPTOMS

History of tick bite or handling animals.

Travel history to endemic area.

Haemorrhagic signs.

Disseminated intravascular coagulation.

May be difficult to distinguish from dengue fever without specific diagnostic testing.

INVESTIGATIONS

Serology or specific viral detection for Crimean-Congo virus: positive.

Lassa fever

SIGNS / SYMPTOMS
INVESTIGATIONS
SIGNS / SYMPTOMS

Exposure to mouse excreta.

Travel history to endemic area.

Haemorrhagic signs.

Encephalopathy.

May be difficult to distinguish from dengue fever without specific diagnostic testing.

INVESTIGATIONS

Serology or specific viral detection for Lassa virus: positive.

Ebola virus infection

SIGNS / SYMPTOMS
INVESTIGATIONS
SIGNS / SYMPTOMS

Travel history to endemic area.

Haemorrhagic signs.

May be difficult to distinguish from dengue fever without specific diagnostic testing.

INVESTIGATIONS

Serology or specific viral detection for Ebola virus: positive.

Marburg virus infection

SIGNS / SYMPTOMS
INVESTIGATIONS
SIGNS / SYMPTOMS

Travel history to endemic area.

Haemorrhagic signs.

May be difficult to distinguish from dengue fever without specific diagnostic testing.

INVESTIGATIONS

Serology or specific viral detection for Marburg virus: positive.

South American haemorrhagic fevers

SIGNS / SYMPTOMS
INVESTIGATIONS
SIGNS / SYMPTOMS

Travel history to endemic area.

Haemorrhagic signs.

May be difficult to distinguish from dengue fever without specific diagnostic testing.

INVESTIGATIONS

Serology or specific viral detection for Junin, Guanarito, Machupo, Chapare, or Sabia virus: positive.

Rift Valley fever

SIGNS / SYMPTOMS
INVESTIGATIONS
SIGNS / SYMPTOMS

Travel history to endemic area.

Haemorrhagic signs.

May be difficult to distinguish from dengue fever without specific diagnostic testing.

INVESTIGATIONS

Serology or specific viral detection for Rift Valley fever virus: positive.

Coronavirus disease 2019 (COVID-19)

SIGNS / SYMPTOMS
INVESTIGATIONS
SIGNS / SYMPTOMS

Important to consider the current epidemiologic situation. May give history of unwell contacts.

Respiratory symptoms may be prominent.

INVESTIGATIONS

Real-time reverse transcription polymerase chain reaction (RT-PCR): positive for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. Rapid antigen tests may also be used.

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