The most common presenting symptom is rectal bleeding and/or pain, which occurs in 45% of patients. Thirty percent of patients report pain or the sensation of a mass.[3]Ryan DP, Compton CC, Mayer RJ. Carcinoma of the anal canal. N Engl J Med. 2000 Mar 16;342(11):792-800.
http://www.ncbi.nlm.nih.gov/pubmed/10717015?tool=bestpractice.com
Anal cancer may also present with itching, discharge, faecal incontinence, fistulae, or a non-healing ulcer.[4]Rao S, Guren MG, Khan K, et al. Anal cancer: ESMO clinical practice guidelines for diagnosis, treatment and follow-up. Ann Oncol. 2021 Sep;32(9):1087-100.
https://www.annalsofoncology.org/article/S0923-7534(21)02064-0/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/34175386?tool=bestpractice.com
Twenty percent of patients have no rectal symptoms.[3]Ryan DP, Compton CC, Mayer RJ. Carcinoma of the anal canal. N Engl J Med. 2000 Mar 16;342(11):792-800.
http://www.ncbi.nlm.nih.gov/pubmed/10717015?tool=bestpractice.com
Commonly, patients delay consulting their physician, and bleeding is often attributed to haemorrhoids.[3]Ryan DP, Compton CC, Mayer RJ. Carcinoma of the anal canal. N Engl J Med. 2000 Mar 16;342(11):792-800.
http://www.ncbi.nlm.nih.gov/pubmed/10717015?tool=bestpractice.com
[4]Rao S, Guren MG, Khan K, et al. Anal cancer: ESMO clinical practice guidelines for diagnosis, treatment and follow-up. Ann Oncol. 2021 Sep;32(9):1087-100.
https://www.annalsofoncology.org/article/S0923-7534(21)02064-0/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/34175386?tool=bestpractice.com
The family physician is usually the first to examine the patient.
History
Key things to ask about in the history include:
Anal trauma
Haemorrhoids
Human papillomavirus (HPV) infection
Sexual history, to determine risk of HIV infection
A family history of colorectal cancer (although there is no relationship between colorectal cancer and anal cancer, this history is important because rectal bleeding can be related to colorectal cancer).
Physical examination and anoscopy
Given the location of the tumour, physical examination is the most important diagnostic and staging procedure. The physical examination should be focused on the inguinal nodes, a rectal examination, and anoscopy.[4]Rao S, Guren MG, Khan K, et al. Anal cancer: ESMO clinical practice guidelines for diagnosis, treatment and follow-up. Ann Oncol. 2021 Sep;32(9):1087-100.
https://www.annalsofoncology.org/article/S0923-7534(21)02064-0/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/34175386?tool=bestpractice.com
The superficial inguinal nodes, medial (deep) inguinal nodes, and nodes close to the pubis should be palpated.[4]Rao S, Guren MG, Khan K, et al. Anal cancer: ESMO clinical practice guidelines for diagnosis, treatment and follow-up. Ann Oncol. 2021 Sep;32(9):1087-100.
https://www.annalsofoncology.org/article/S0923-7534(21)02064-0/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/34175386?tool=bestpractice.com
Most inguinal lymph node metastases are unilateral and ipsilateral to the primary tumour.[18]Gerard JP, Chapet O, Samiei F, et al. Management of inguinal lymph node metastases in patients with carcinoma of the anal canal: experience in a series of 270 patients treated in Lyon and review of the literature. Cancer. 2001 Jul 1;92(1):77-84.
https://www.doi.org/10.1002/1097-0142(20010701)92:1<77::aid-cncr1294>3.0.co;2-p
http://www.ncbi.nlm.nih.gov/pubmed/11443612?tool=bestpractice.com
Female patients should have a gynaecological examination to assess the primary tumour and exclude vaginal invasion or fistula formation.[4]Rao S, Guren MG, Khan K, et al. Anal cancer: ESMO clinical practice guidelines for diagnosis, treatment and follow-up. Ann Oncol. 2021 Sep;32(9):1087-100.
https://www.annalsofoncology.org/article/S0923-7534(21)02064-0/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/34175386?tool=bestpractice.com
Benign haemorrhoidal bleeding is a diagnosis of exclusion.
The UK National Institute for Health and Care Excellence (NICE) recommends that patients aged 50 years or older, who have unexplained rectal bleeding, should be referred for an urgent (2-week wait) hospital appointment.[31]National Institute for Health and Care Excellence. Suspected cancer: recognition and referral. Aug 2023 [internet publication].
https://www.nice.org.uk/guidance/ng12
Clinicians should consider urgent referral for patients who:[31]National Institute for Health and Care Excellence. Suspected cancer: recognition and referral. Aug 2023 [internet publication].
https://www.nice.org.uk/guidance/ng12
Are aged under 50 years and have rectal bleeding with any of the following: abdominal pain, change in bowel habit, weight loss, iron deficiency anaemia
Have an unexplained anal mass or anal ulceration
Have a rectal mass.
Biopsy
All suspicious anal lesions should be excised or biopsied. Biopsy is essential to confirm the diagnosis.[4]Rao S, Guren MG, Khan K, et al. Anal cancer: ESMO clinical practice guidelines for diagnosis, treatment and follow-up. Ann Oncol. 2021 Sep;32(9):1087-100.
https://www.annalsofoncology.org/article/S0923-7534(21)02064-0/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/34175386?tool=bestpractice.com
An incisional biopsy is recommended for diagnosis. Excisional biopsies should be limited to small superficial lesions.
Fine needle aspiration or excisional biopsy of clinically or radiologically enlarged inguinal lymph nodes is recommended.[8]National Comprehensive Cancer Network. NCCN clinical practice guidelines in oncology: anal carcinoma [internet publication].
https://www.nccn.org/professionals/physician_gls/default.aspx
A formal inguinal lymph node dissection is not recommended because of the associated morbidity, its failure to have an impact on outcome, and the high control rates achieved with chemoradiation.[32]Lin ACH, Hakim A, Kellish AS, et al. Inguinal lymph node dissection does not improve overall survival in anal cancer nodal disease. J Surg Res. 2020 Nov;255:13-22.
http://www.ncbi.nlm.nih.gov/pubmed/32540576?tool=bestpractice.com
Radiological imaging
High resolution T2-weighted magnetic resonance imaging (MRI) or computed tomography (CT) of the pelvis should be performed to assess the primary tumour and pelvic lymph nodes, and to evaluate whether the tumour involves other organs.[4]Rao S, Guren MG, Khan K, et al. Anal cancer: ESMO clinical practice guidelines for diagnosis, treatment and follow-up. Ann Oncol. 2021 Sep;32(9):1087-100.
https://www.annalsofoncology.org/article/S0923-7534(21)02064-0/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/34175386?tool=bestpractice.com
[8]National Comprehensive Cancer Network. NCCN clinical practice guidelines in oncology: anal carcinoma [internet publication].
https://www.nccn.org/professionals/physician_gls/default.aspx
US guidelines advise using either CT with contrast or MRI with contrast to evaluate the pelvis.[8]National Comprehensive Cancer Network. NCCN clinical practice guidelines in oncology: anal carcinoma [internet publication].
https://www.nccn.org/professionals/physician_gls/default.aspx
European guidelines recommend using MRI pelvis for assessment of the primary tumour and CT pelvis with contrast to screen for metastatic disease.[4]Rao S, Guren MG, Khan K, et al. Anal cancer: ESMO clinical practice guidelines for diagnosis, treatment and follow-up. Ann Oncol. 2021 Sep;32(9):1087-100.
https://www.annalsofoncology.org/article/S0923-7534(21)02064-0/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/34175386?tool=bestpractice.com
A CT scan of the abdomen and chest should be also performed to screen for metastases.[4]Rao S, Guren MG, Khan K, et al. Anal cancer: ESMO clinical practice guidelines for diagnosis, treatment and follow-up. Ann Oncol. 2021 Sep;32(9):1087-100.
https://www.annalsofoncology.org/article/S0923-7534(21)02064-0/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/34175386?tool=bestpractice.com
[8]National Comprehensive Cancer Network. NCCN clinical practice guidelines in oncology: anal carcinoma [internet publication].
https://www.nccn.org/professionals/physician_gls/default.aspx
Staging (18F)-fluoro-2-deoxy-D-glucose (FDG)-positron emission tomography (PET)/CT and PET/MRI scans should be considered, in addition to standard diagnostic imaging with CT or MRI.[8]National Comprehensive Cancer Network. NCCN clinical practice guidelines in oncology: anal carcinoma [internet publication].
https://www.nccn.org/professionals/physician_gls/default.aspx
PET/CT has a sensitivity of 99% for the detection of primary disease and a sensitivity of 93% for the detection of inguinal lymph node involvement.[33]Mahmud A, Poon R, Jonker D. PET imaging in anal canal cancer: a systematic review and meta-analysis. Br J Radiol. 2017 Dec;90(1080):20170370.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6047643
http://www.ncbi.nlm.nih.gov/pubmed/28972796?tool=bestpractice.com
[34]Jones M, Hruby G, Solomon M, et al. The role of FDG-PET in the initial staging and response assessment of anal cancer: a systematic review and meta-analysis. Ann Surg Oncol. 2015 Oct;22(11):3574-81.
http://www.ncbi.nlm.nih.gov/pubmed/25652048?tool=bestpractice.com
In one meta-analysis, PET/CT led to a change in nodal staging in 28% of patients.[34]Jones M, Hruby G, Solomon M, et al. The role of FDG-PET in the initial staging and response assessment of anal cancer: a systematic review and meta-analysis. Ann Surg Oncol. 2015 Oct;22(11):3574-81.
http://www.ncbi.nlm.nih.gov/pubmed/25652048?tool=bestpractice.com
CT-based simulation is conducted for radiotherapy planning. FDG-PET/CT, FDG-PET/MRI, and MRI pelvis results may also influence planning, in particular dose or field changes.[8]National Comprehensive Cancer Network. NCCN clinical practice guidelines in oncology: anal carcinoma [internet publication].
https://www.nccn.org/professionals/physician_gls/default.aspx
[33]Mahmud A, Poon R, Jonker D. PET imaging in anal canal cancer: a systematic review and meta-analysis. Br J Radiol. 2017 Dec;90(1080):20170370.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6047643
http://www.ncbi.nlm.nih.gov/pubmed/28972796?tool=bestpractice.com
Screening for associated conditions
HIV testing is recommended for patients with anal cancer whose HIV status is unknown, and for people with multifocal or recurrent anal intraepithealial neoplasia.[4]Rao S, Guren MG, Khan K, et al. Anal cancer: ESMO clinical practice guidelines for diagnosis, treatment and follow-up. Ann Oncol. 2021 Sep;32(9):1087-100.
https://www.annalsofoncology.org/article/S0923-7534(21)02064-0/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/34175386?tool=bestpractice.com
[8]National Comprehensive Cancer Network. NCCN clinical practice guidelines in oncology: anal carcinoma [internet publication].
https://www.nccn.org/professionals/physician_gls/default.aspx
Female patients with anal intraepithealial neoplasia or anal cancer should be offered screening for synchronocus cervical epithelial neoplasia, vulvar intraepithealial neoplasia, and vaginal intraepithealial neoplasia, which are also associated with HPV.[4]Rao S, Guren MG, Khan K, et al. Anal cancer: ESMO clinical practice guidelines for diagnosis, treatment and follow-up. Ann Oncol. 2021 Sep;32(9):1087-100.
https://www.annalsofoncology.org/article/S0923-7534(21)02064-0/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/34175386?tool=bestpractice.com