Criteria

Criteria for the diagnosis of Coxiella burnetii primary infection based on acute symptoms and history of valvulopathy, immunodeficiency, or pregnancy[45]

Acute Q fever:

  • Fever, hepatitis and/or pneumonia with microbiological criteria (phase II IgG ≥1:200 and phase II IgM ≥1:50, seroconversion, or a positive polymerase chain reaction [PCR] on blood/serum and no endocarditis)

  • Duration of symptoms <3 months after symptoms onset or seroconversion.

Acute Q fever with significant valvulopathy:

  • Criteria for acute Q fever plus history of rheumatic fever, bicuspid aortic valve, congenital heart disease, prosthetic heart valves, valve regurgitation, stenosis grade ≥II, mitral valve prolapse.

Acute Q fever with significant vasculopathy:

  • Criteria for acute Q fever plus history of vascular graft or vascular aneurysm.

Acute Q fever with severe immunodeficiency:

  • Criteria for acute Q fever in transplant patients, patients undergoing chemotherapy or corticosteroid therapy, patients with HIV with <200 CD4, patients with haematological malignancies.

Asymptomatic primary infection with C burnetii during pregnancy:

  • Asymptomatic pregnant woman with both phase II IgG ≥1:200 and IgM ≥1:50.

Criteria for the diagnosis of C burnetii endocarditis[82]

A. Definite criteria:

  • Positive culture

  • PCR or immunochemistry of a cardiac valve.

B. Major criteria:

  • Microbiology: positive culture or PCR of the blood or emboli, or serology with phase I IgG antibodies ≥1:6400

  • Evidence of endocardial involvement:

    • Echocardiogram positive for infective endocarditis: oscillating intracardiac mass on valve or supporting structures, in the path of regurgitant jets, or on implanted material in the absence of an alternative anatomical explanation; or abscess; or new partial dehiscence of prosthetic valve; or new valvular regurgitation (worsening or changing of pre-existing murmur not sufficient)

  • PET scan showing a specific valve fixation and mycotic aneurysm.

C. Minor criteria:

  • Predisposing heart condition (known or found on echography)

  • Fever (>38°C)

  • Vascular phenomena, major arterial emboli, septic pulmonary infarcts, mycotic aneurysm (at PET scan), intracranial haemorrhage, conjunctival haemorrhages, and Janeway’s lesions

  • Immunological phenomena: glomerulonephritis, Osler’s nodes, Roth's spots, or rheumatoid factor

  • Positive serology with phase I IgG antibodies ≥1:800 and <1:6400.

Definite diagnosis:

  1. One A criterion

  2. Two B criteria

  3. One B criterion and three C criteria (including evidence of microbiology and cardiac predisposition).

Possible diagnosis:

  1. One B criterion and two C criteria (including evidence of microbiology and cardiac predisposition)

  2. Three C criteria (including positive serology and cardiac predisposition).

Patients meeting the criteria for definite or possible C burnetii endocarditis on prosthetic heart valve or pacemaker, or following Bentall surgery, should be diagnosed as having definite or possible foreign body-related endocarditis, respectively, and should be treated accordingly (i.e., 24-month treatment course).

Patients with severe heart valve disease (generally in a cardiac surgery unit) have been diagnosed with definite endocarditis with phase I IgG levels as low as 1:200.[55][58] In this specific context (cardiac surgery and vascular surgery and very low serological titres between 1:200 and 1:400), treatment of endocarditis and vascular infection must be prescribed even in the absence of infectious symptoms or a positive PCR since mortality risk is high if left untreated.

Criteria for diagnosis of C burnetii vascular infection[82]

A. Definite criteria:

  • Positive culture

  • PCR or immunochemistry of an arterial sample (prosthesis or aneurysm) or a periarterial abscess, or a spondylodiscitis linked to aorta.

B. Major criteria:

  • Microbiology: positive culture, PCR of the blood or emboli, or serology with phase I IgG antibodies ≥1:6400

  • Evidence of vascular involvement:

    • CT scan: aneurysm or vascular prosthesis, and periarterial abscess, fistula, or spondylodiscitis

    • PET scan: specific fixation on an aneurysm or vascular prosthesis.

C. Minor criteria:

  • Positive serology with phase I IgG antibodies ≥1:800 and <1:6400

  • Fever (≥38°C)

  • Emboli

  • Underlying vascular predisposition (e.g., aneurysm or vascular prosthesis).

Definite diagnosis:

  1. One A criterion

  2. Two B criteria

  3. One B criterion and two C criteria (including evidence of microbiology and vascular predisposition).

Possible diagnosis:

  • Vascular predisposition, serological evidence, and fever or emboli.

Patients meeting the criteria for definite or possible C burnetii vascular infection on vascular prosthetic material should be diagnosed as having definite or possible foreign-body related vascular infection, and should be treated accordingly (i.e., 24-month treatment course).

Criteria for diagnosis of C burnetii prosthetic joint infection[83]

A. Definite criteria:

  • Positive culture

  • PCR or immunochemistry of a periprosthetic biopsy or joint aspirate.

B. Major criteria:

  • Microbiology:

    • Positive culture, or PCR of the blood

    • Positive C burnetii serology with phase I IgG antibodies ≥1:6400

  • Evidence of prosthetic involvement:

    • CT scan or MRI positive for prosthetic infection: collection or pseudotumour of the prosthesis

    • PET scan or indium leukocyte scan showing a specific prosthetic hypermetabolism consistent with infection

      • For the 18F-fluorodeoxyglucose PET scan, the uptake at the bone-prosthesis interface with exclusion of the head and tip is considered the best criterion for infection, with 92% sensitivity and 97% specificity.

C. Minor criteria:

  • Presence of a joint prosthesis (indispensable criteria)

  • Fever (≥38°C)

  • Joint pain

  • Positive serology with phase I IgG antibodies >1:800 and <1:6400.

Definite diagnosis:

  1. One A criterion

  2. Two B criteria

  3. One B criterion and three C criteria (including evidence of microbiology and presence of a joint prosthesis).

Possible diagnosis:

  1. One B criterion and two C criteria (including evidence of microbiology and presence of a joint prosthesis)

  2. Three C criteria (including positive serology and presence of a joint prosthesis).

Criteria for diagnosis of C burnetii osteoarticular infection without prosthesis[3][78]

A. Definite criteria:

  • Positive culture

  • PCR or immunochemistry of bone or synovial biopsy or joint aspirate.

B. Major criteria:

  • Microbiology:

    • Positive culture or positive PCR of the blood

    • Positive serology with phase I IgG antibodies ≥1:800

  • Evidence of bone or joint involvement:

    • Clinical arthritis, osteitis, or tenosynovitis

    • CT scan or ultrasonography (for joint) or MRI: osteoarticular destruction, joint effusion, intra-articular collection, spondylodiscitis, synovitis, acromioclavicular localisation

    • PET scan or indium leukocyte scan showing a specific osteoarticular uptake.

C. Minor criteria:

  • Positive serology with phase I IgG antibodies ≥1:400 and <1:800

  • Fever (≥38°C)

  • Mono- or polyarthralgia.

Definite diagnosis:

  1. One A criterion

  2. Two B criteria

  3. One B criterion and three C criteria (including one microbiological characteristic).

Possible diagnosis:

  1. One B criterion and two C criteria

  2. Three C criteria.

Criteria for diagnosis of C burnetii chronic lymphadenitis[3][78]

A. Definite criteria:

  • Positive culture

  • PCR or immunohistochemistry or fluorescence in situ hybridisation (FISH) of lymphadenitis.

B. Major criteria:

  • Microbiology:

    • Positive culture, or positive PCR of the blood

    • Positive serology with phase I IgG antibodies ≥1:800

  • Evidence of lymph node involvement:

    • Clinical lymphadenitis

    • CT scan or ultrasonography (for joint) or MRI: lymphadenitis >1 cm

    • PET scan showing specific lymph node uptake.

C. Minor criteria:

  • Positive serology with phase I IgG antibodies ≥1:400 and <1:800

  • Fever (≥38°C).

Definite diagnosis:

  1. One A criterion

  2. Two B criteria

  3. One B criterion and two C criteria (including one microbiological characteristic).

Possible diagnosis:

  1. One B criterion and one C criterion

  2. Two C criteria.

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