Tests

1st tests to order

Helicobacter pylori urea breath test or stool antigen test

Test
Result
Test

Ordered in patients aged <60 years, even in the presence of alarm symptoms.

Proton-pump inhibitors (PPIs), bismuth, and antibiotics can interfere with the performance of diagnostic tests for Helicobacter pylori.

Note that no test for H pylori is 100% sensitive. The use of a second test may be considered if an ulcer is found in a patient without H pylori.

Result

positive result if H pylori present

upper gastrointestinal endoscopy

Test
Result
Test

The most specific and sensitive test. Initially ordered if the patient presenting with dyspeptic symptoms is aged ≥60 years. May also be considered subsequent to treatment if the patient fails to respond.

If an ulcer is present, Helicobacter pylori testing should always be performed. Histology and biopsy urease testing (rapid urease test) are performed on stomach biopsies obtained during endoscopy. Both tests can detect H pylori; however, histology can determine if the ulcer is neoplastic (very rarely) and/or if there is evidence of a nonsteroidal anti-inflammatory drug (NSAID) being the likely cause.

Endoscopy should be repeated after 6 to 8 weeks in patients with gastric ulcer to ensure ulcer healing and to rule out malignancy (unless excluded at initial endoscopy).

If the patient presents with upper GI bleeding, endoscopy is usually carried out first-line; however, if endoscopy is not possible, CT angiography (CTA) abdomen and pelvis or visceral angiography are alternatives that may be considered depending on the stability of the patient to undergo these procedures.[55]

Result

peptic ulcer; may also detect cause (e.g., H pylori)

CBC

Test
Result
Test

Ordered only if the patient seems clinically anemic or has evidence of gastrointestinal bleeding.

Aids in evaluating the urgency with which the patient requires further workup, but does not definitively diagnose peptic ulcer disease.

Result

microcytic anemia

Investigations to avoid

Serology

Recommendations
Rationale
Recommendations

Do not order serologic antibody tests when testing for active H pylori infection.[51]

Rationale

Specificity and sensitivity of H pylori serology is lower than other noninvasive tests such as stool antigen or carbon-urea breath testing. A positive result for an IgG H pylori antibody test does not indicate an active infection.[49][50]​​[52][53]​​​

Tests to consider

fasting serum gastrin level

Test
Result
Test

Ordered if there are multiple duodenal ulcers (especially postbulbar) or in patient with ulcers and diarrhea.

Patient must be fasting and proton-pump inhibitor therapy stopped.

Elevated levels in pernicious anemia and other hypochlorhydric states results in low specificity.

Result

hypergastrinemia in Zollinger-Ellison syndrome

CTA abdomen and pelvis without and with contrast

Test
Result
Test

If the patient presents with upper GI bleeding, endoscopy is usually carried out first-line; however, if endoscopy is not possible, CTA abdomen and pelvis or visceral angiography are alternatives that may be considered depending on the stability of the patient to undergo these procedures.[55][56]

Result

may localize bleeding site

arteriography visceral

Test
Result
Test

If the patient presents with upper GI bleeding, endoscopy is usually carried out first-line; however, if endoscopy is not possible CTA abdomen and pelvis or visceral angiography are alternatives that may be considered depending on the stability of the patient to undergo these procedures.[55]​ A limitation of this test is that venous bleeding can be missed because the contrast resolution of the venous phase of an angiogram is relatively poor.[55]

Result

detects bleeding if the rate of bleeding is ≥0.5 mL/min

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