Consensus recommendations categorise HP into non-fibrotic and fibrotic clinical phenotypes, based on the presence or absence of fibrosis on high-resolution computed tomography (HRCT).[3]Raghu G, Remy-Jardin M, Ryerson CJ, et al. Diagnosis of hypersensitivity pneumonitis in adults. An Official ATS/JRS/ALAT clinical practice guideline. Am J Respir Crit Care Med. 2020 Aug 1;202(3):e36-69.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397797
http://www.ncbi.nlm.nih.gov/pubmed/32706311?tool=bestpractice.com
[33]Fernández Pérez ER, Travis WD, Lynch DA, et al. Diagnosis and evaluation of hypersensitivity pneumonitis: CHEST guideline and expert panel report. Chest. 2021 Aug;160(2):e97-156.
https://journal.chestnet.org/article/S0012-3692(21)00686-3/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/33861992?tool=bestpractice.com
However, no one test is pathognomonic.
Clinicians should instead conduct a thorough history to identify inciting antigens, including occupational exposure, and to establish the type, extent, and temporal relationship of exposure(s) to symptoms.[33]Fernández Pérez ER, Travis WD, Lynch DA, et al. Diagnosis and evaluation of hypersensitivity pneumonitis: CHEST guideline and expert panel report. Chest. 2021 Aug;160(2):e97-156.
https://journal.chestnet.org/article/S0012-3692(21)00686-3/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/33861992?tool=bestpractice.com
Note that a substantial minority of patients will be unable to provide a history of exposure. A 2020 Delphi expert group suggests inquiring about occult exposures (e.g., feather pillows/cushions/duvets or mould beneath floorboards).[34]Barnes H, Morisset J, Molyneaux P, et al. A systematically derived exposure assessment instrument for chronic hypersensitivity pneumonitis. Chest. 2020 Jun;157(6):1506-12.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7268442
http://www.ncbi.nlm.nih.gov/pubmed/31958445?tool=bestpractice.com
Clinical improvement with antigen avoidance or therapy usually supports the diagnosis, but a lack of improvement is not sufficient alone to exclude the diagnosis. Possible corroborative tests include abnormal chest x-ray or computed tomography (CT), a high titre antibody against the suspected antigen, and abnormal pulmonary function tests (PFTs).[3]Raghu G, Remy-Jardin M, Ryerson CJ, et al. Diagnosis of hypersensitivity pneumonitis in adults. An Official ATS/JRS/ALAT clinical practice guideline. Am J Respir Crit Care Med. 2020 Aug 1;202(3):e36-69.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397797
http://www.ncbi.nlm.nih.gov/pubmed/32706311?tool=bestpractice.com
[33]Fernández Pérez ER, Travis WD, Lynch DA, et al. Diagnosis and evaluation of hypersensitivity pneumonitis: CHEST guideline and expert panel report. Chest. 2021 Aug;160(2):e97-156.
https://journal.chestnet.org/article/S0012-3692(21)00686-3/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/33861992?tool=bestpractice.com
Clinical assessment
Common symptoms of both non-fibrotic and fibrotic HP include dyspnoea and cough.[3]Raghu G, Remy-Jardin M, Ryerson CJ, et al. Diagnosis of hypersensitivity pneumonitis in adults. An Official ATS/JRS/ALAT clinical practice guideline. Am J Respir Crit Care Med. 2020 Aug 1;202(3):e36-69.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397797
http://www.ncbi.nlm.nih.gov/pubmed/32706311?tool=bestpractice.com
[33]Fernández Pérez ER, Travis WD, Lynch DA, et al. Diagnosis and evaluation of hypersensitivity pneumonitis: CHEST guideline and expert panel report. Chest. 2021 Aug;160(2):e97-156.
https://journal.chestnet.org/article/S0012-3692(21)00686-3/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/33861992?tool=bestpractice.com
There may also be mid inspiratory squeaks and cyanosis on physical examination. Systemic symptoms include weight loss, flu-like symptoms (e.g, malaise, chills, and low-grade fever), chest tightness, and wheezing.[24]Lacasse Y, Selman M, Costabel U, et al. Clinical diagnosis of hypersensitivity pneumonitis. Am J Respir Crit Care Med. 2003 Oct 15;168(8):952-8.
http://www.atsjournals.org/doi/full/10.1164/rccm.200301-137OC
http://www.ncbi.nlm.nih.gov/pubmed/12842854?tool=bestpractice.com
Onset may be acute (developing over days to weeks; more usually associated with non-fibrotic HP), or insidious (developing and worsening over months to years; more frequently seen with fibrotic HP). Episodes may be recurrent. Patients with fibrotic HP are more likely to be older and have unknown exposure to a causative antigen.[35]De Sadeleer LJ, Hermans F, De Dycker E, et al. Effects of corticosteroid treatment and antigen avoidance in a large hypersensitivity pneumonitis cohort: a single-centre cohort study. J Clin Med. 2019 Jan; 8(1): 14.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352061
http://www.ncbi.nlm.nih.gov/pubmed/30577667?tool=bestpractice.com
Recurrent flu-like illness or interstitial lung disease should raise a high index of suspicion for HP.
Laboratory
Full blood count, erythrocyte sedimentation rate (ESR), and albumin are of limited value but may indicate an inflammatory process and/or chronic disease process. In acute settings, there may be non-specific findings such as an elevated ESR and leukocytosis with a left shift. In chronic conditions, there are often non-specific findings such as low albumin and anaemia.[36]McSharry C, Anderson K, Boyd G. A review of antigen diversity causing lung disease among pigeon breeders. Clin Exp Allergy. 2000 Sep;30(9):1221-9.
http://www.ncbi.nlm.nih.gov/pubmed/10971467?tool=bestpractice.com
[37]Schenker M. Exposures and health effects from inorganic agricultural dusts. Environ Health Perspect. 2000 Aug;108 Suppl 4:661-4.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1637665/pdf/envhper00313-0060.pdf
http://www.ncbi.nlm.nih.gov/pubmed/10931784?tool=bestpractice.com
High titres against serum antigen-specific immunoglobulin G (IgG) or immunoglobulin A (IgA) are corroborative.
Imaging
The chest x-ray in acute presentations and non-fibrotic disease is generally characterised by patchy, nodular infiltrates, while in later stages there is fibrosis and peribronchial thickening.[38]Khalil N, Churg A, Muller N, et al. Environmental, inhaled and ingested causes of pulmonary fibrosis. Toxicol Pathol. 2007 Jan;35(1):86-96.
http://www.ncbi.nlm.nih.gov/pubmed/17325977?tool=bestpractice.com
However, chest x-ray may be normal between episodes in non-fibrotic disease and the chest x-ray findings are non-specific; therefore, patients with suspected HP require a HRCT.[33]Fernández Pérez ER, Travis WD, Lynch DA, et al. Diagnosis and evaluation of hypersensitivity pneumonitis: CHEST guideline and expert panel report. Chest. 2021 Aug;160(2):e97-156.
https://journal.chestnet.org/article/S0012-3692(21)00686-3/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/33861992?tool=bestpractice.com
In non-fibrotic HP, the HRCT findings include bilateral, diffuse ground-glass opacities and mosaic attenuation, plus at least one of the following features suggestive of small airways disease:
Non-specific features that are compatible with non-fibrotic HP include: uniform and subtle ground glass opacities, airspace consolidation, and lung cysts.
In fibrotic HP, the HRCT findings most commonly consist of signs of lung fibrosis (e.g., irregular linear opacities or coarse reticulation) with lung distortion and possible septal thickening. The pattern of fibrosis may be mid-zone predominant, mid- to lower-zone, or distributed throughout the lung with relative basal sparing. Traction bronchiectasis and honeycombing may also be present.
The 'three-density pattern' (formerly called the 'headcheese sign') is specific to fibrotic HP, with sharply demarcated areas of:
high attenuation (ground-glass opacity)
regions of decreased attenuation and vascularity
normal lung.[3]Raghu G, Remy-Jardin M, Ryerson CJ, et al. Diagnosis of hypersensitivity pneumonitis in adults. An Official ATS/JRS/ALAT clinical practice guideline. Am J Respir Crit Care Med. 2020 Aug 1;202(3):e36-69.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397797
http://www.ncbi.nlm.nih.gov/pubmed/32706311?tool=bestpractice.com
Physiological
PFTs are typically abnormal, demonstrating either restriction or obstruction, or both, and diffusing lung capacity of carbon monoxide (DLCO) is reduced. PFTs and DLCO may be normal between acute attacks.
Bronchoalveolar lavage may occasionally be necessary to confirm the diagnosis or exclude differentials.[3]Raghu G, Remy-Jardin M, Ryerson CJ, et al. Diagnosis of hypersensitivity pneumonitis in adults. An Official ATS/JRS/ALAT clinical practice guideline. Am J Respir Crit Care Med. 2020 Aug 1;202(3):e36-69.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397797
http://www.ncbi.nlm.nih.gov/pubmed/32706311?tool=bestpractice.com
[33]Fernández Pérez ER, Travis WD, Lynch DA, et al. Diagnosis and evaluation of hypersensitivity pneumonitis: CHEST guideline and expert panel report. Chest. 2021 Aug;160(2):e97-156.
https://journal.chestnet.org/article/S0012-3692(21)00686-3/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/33861992?tool=bestpractice.com
[39]Meyer KC, Raghu G, Baughman RP, et al. An official American Thoracic Society clinical practice guideline: the clinical utility of bronchoalveolar lavage cellular analysis in interstitial lung disease. Am J Respir Crit Care Med. 2012 May 1;185(9):1004-14.
http://www.atsjournals.org/doi/full/10.1164/rccm.201202-0320ST#.VRKPZI6Dkyo
http://www.ncbi.nlm.nih.gov/pubmed/22550210?tool=bestpractice.com
Compared with patients with non-fibrotic HP, those with fibrotic HP usually have a lower vital capacity and diffusion capacity, and a lower percentage of lymphocytes in their bronchoalveolar lavage fluid.[3]Raghu G, Remy-Jardin M, Ryerson CJ, et al. Diagnosis of hypersensitivity pneumonitis in adults. An Official ATS/JRS/ALAT clinical practice guideline. Am J Respir Crit Care Med. 2020 Aug 1;202(3):e36-69.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397797
http://www.ncbi.nlm.nih.gov/pubmed/32706311?tool=bestpractice.com
Antigen-specific inhalation challenges are rarely used and potentially dangerous.[33]Fernández Pérez ER, Travis WD, Lynch DA, et al. Diagnosis and evaluation of hypersensitivity pneumonitis: CHEST guideline and expert panel report. Chest. 2021 Aug;160(2):e97-156.
https://journal.chestnet.org/article/S0012-3692(21)00686-3/fulltext
http://www.ncbi.nlm.nih.gov/pubmed/33861992?tool=bestpractice.com
Biopsy
Lung biopsy may occasionally be performed when a multidisciplinary team cannot make a diagnosis based on clinical, laboratory, or imaging data (e.g., patients with atypical features or no history of exposure).[40]Korevaar DA, Colella S, Fally M, et al. European Respiratory Society guidelines on transbronchial lung cryobiopsy in the diagnosis of interstitial lung diseases. Eur Respir J. 2022 Nov 10;60(5):2200425.
https://erj.ersjournals.com/content/60/5/2200425.long
http://www.ncbi.nlm.nih.gov/pubmed/35710261?tool=bestpractice.com
Transbronchial lung cryobiopsy is an option where surgical lung biopsy is not suitable. Typical histological features include bronchocentric infiltrate consisting of lymphocytes, plasma cells, neutrophils, foamy macrophages, and non-caseating granulomas. In later stages, interstitial fibrosis predominates.