Differentials

Common

Bacterial vaginosis

History

often asymptomatic; fishy odour especially after condomless intercourse; off-white, thin, homogeneous discharge; rarely dysuria and dyspareunia; risk factors including new sexual partner or >3 in past year, douching, cigarette smoking

Exam

discharge typically homogeneous, thin, grayish-white and odorous

1st investigation
  • Amsel's criteria:

    at least 3 out of 4 of: thin, homogeneous discharge; vaginal pH >4.5; a positive whiff test or release of amine odour with the addition of base (10% potassium hydroxide); clue cells on microscopic evaluation of saline wet preparation

  • KOH test of vaginal discharge:

    presence of fishy odour when 10% potassium hydroxide (KOH) is added to vaginal discharge

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  • wet mount microscopy of vaginal discharge:

    clue cells

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  • Gram stain:

    relative concentration of lactobacilli

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Other investigations
  • nucleic acid amplification test:

    positive for specific organism

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  • nucleic acid probe:

    positive for typical organisms

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  • card test:

    detection of pH, trimethylamine, and proline aminopeptidase

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Trichomoniasis

History

purulent, malodorous, thin discharge; can also present with burning, pruritus, dysuria, frequency, and dyspareunia; symptoms may be worse during menstruation

Exam

typically, erythema of the vulva and vaginal mucosa; vaginal discharge (green-yellow, frothy) and strawberry cervix are not reliable clinical signs but may be present

1st investigation
  • nucleic acid amplification test:

    positive for Trichomonas vaginalis

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  • rapid antigen test:

    positive

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  • wet mount microscopy of vaginal discharge:

    positive for Trichomonas vaginalis

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  • nucleic acid probe:

    positive for Trichomonas vaginalis

Other investigations
  • culture from vaginal sample on Diamond medium:

    positive

    More

Vulvovaginal candidiasis

History

vulvar pruritus, dysuria, pain, burning, swelling, redness, soreness, irritation, dyspareunia; usually little or no discharge but if present, appears white and clumpy, curd-like; more frequent in patients with diabetes

Exam

erythema of the vulva, vaginal mucosa, and vulva oedema; with Candida albicans discharge usually thick, adherent, cottage cheese-like, but may be thin and loose; with Candida glabrata usually little discharge

1st investigation
  • vaginal discharge pH:

    pH 4.0-4.5

  • wet mount microscopy of vaginal discharge:

    budding yeast and hyphae

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  • vaginal fungal culture:

    Candida

    More
Other investigations
  • nucleic acid amplification test:

    positive for Candida

    More
  • nucleic acid probe:

    positive for Candida

  • serum fasting glucose:

    may confirm diabetes in recurrent or resistant disease

  • HbA1c:

    may confirm diabetes in recurrent or resistant disease

Chlamydia trachomatis infection

History

often asymptomatic; or purulent or mucopurulent discharge from endocervix, intermenstrual or postcoital bleeding, dysuria, urinary frequency, dyspareunia, vulvovaginal irritation; pain and fever rare

Exam

cervix friable, erythematous and oedematous, with purulent or mucopurulent discharge; possible cervical motion tenderness; with Chlamydia trachomatis: yellow opaque endocervical discharge, easily induced cervical bleeding​

1st investigation
  • nucleic acid amplification test:

    positive

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Other investigations
  • Chlamydia trachomatis culture:

    positive

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  • Chlamydia trachomatis antigen detection:

    positive

  • DNA cervical or urethral probe:

    positive for Chlamydia trachomatis

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Neisseria gonorrhoeae infection

History

asymptomatic; or vaginal pruritus and/or a mucopurulent discharge; abdominal pain or dyspareunia suggests extension to upper tract; may lead to pelvic inflammatory disease, ectopic pregnancy, infertility if untreated

Exam

cervix normal or with friable mucosa and purulent discharge

1st investigation
  • nucleic acid amplification test:

    positive

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  • DNA cervical or urethral probe:

    positive

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  • culture cervical sample on modified Thayer-Martin medium:

    positive

    More
Other investigations
  • enzyme immunoassay of cervical or urine sample:

    positive

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Mycoplasma genitalium

History

often asymptomatic; or purulent or mucopurulent discharge from endocervix, intermenstrual or postcoital bleeding, dysuria, urinary frequency, dyspareunia, vulvovaginal irritation; abdominopelvic pain

Exam

cervix friable, erythematous with purulent or mucopurulent discharge; possible cervical motion tenderness; easily induced cervical bleeding

1st investigation
  • nucleic acid amplification test:

    positive

    More
Other investigations

    Irritant and allergic vaginitis

    History

    vaginal itching and discharge in association with use of topical medications, spermicidal products, douching solutions, condoms, or diaphragms; can be reaction to sperm, latex, dyes, soap, tampons, pads

    Exam

    vulvar erythema, non-specific vaginal discharge

    1st investigation
    • none:

      diagnosis is clinical

      More
    Other investigations
    • patch testing:

      may be positive for possible irritants

    Physiological discharge in adults

    History

    usually consists of 1 to 5 mL discharge per 24 hours; typically transparent, odourless (but can also be slightly malodorous), mucousy, and white-to-yellowish; more noticeable with higher oestrogen states (e.g., during pregnancy, when using oestrogen-progestin contraceptives, or at ovulation)

    Exam

    scant transparent, mucousy, and white-to-yellowish discharge

    1st investigation
    • microscopy of vaginal sample:

      sheets of vaginal epithelial cells

    Other investigations

      Foreign body in children

      History

      discharge usually bloody and foul-smelling

      Exam

      foreign body, bloody and purulent discharge

      1st investigation
      • abdominal or pelvic x-ray:

        foreign body visualised

      Other investigations
      • pelvic examination (may have to be under anaesthesia):

        foreign body visualised

      Non-specific vaginitis

      History

      irritation from bubble baths, perfumed soaps, tight-fitting clothes, back-to-front wiping, poor wiping after toilet training can lead to non-specific vaginitis; vulvar skin easily traumatised

      Exam

      scant to copious foul-smelling discharge

      1st investigation
      • none:

        diagnosis is clinical (after exclusion of other causes)

      Other investigations

        Physiological discharge in children

        History

        a few months before menarche (9-13 years old); grey-white physiological discharge due to increase in oestrogen levels

        Exam

        scant to moderate, clear to white discharge; otherwise normal

        1st investigation
        • microscopy of vaginal sample:

          sheets of vaginal epithelial cells

        Other investigations

          Uncommon

          Herpes simplex virus (HSV) infection

          History

          superficial sores or ulcers over the vulva; sometimes watery vaginal discharge appears 7-11 days after primary infection; general malaise and fever possible; central nervous system involvement rare

          Exam

          multiple crops of painful, shallow ulcers over the vulva, vagina and cervix, which often coalesce then heal spontaneously without scarring

          1st investigation
          • nucleic acid amplification test:

            positive

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          Other investigations
          • viral cultures of lesions:

            virus detected

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          Streptococcal vaginitis in adults

          History

          predisposing factor present: household or personal history of upper respiratory tract infection with group A streptococci; sexual contact; vaginal atrophy

          Exam

          profuse/copious vaginal discharge

          1st investigation
          • vaginal culture swab:

            positive for Streptococcus pyogenes (group A streptococci)

          Other investigations

            Genital schistosomiasis

            History

            living in endemic area; may be encountered in the West subsequent to increased tourism

            Exam

            erythematous cervix; copious vaginal discharge

            1st investigation
            • microscopy of urine for parasites:

              eggs present

            Other investigations
            • cervical punch biopsy:

              eggs present

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            • high-magnification colposcopy:

              sandy patches with yellow colour on mucosal surfaces

            Entamoeba gingivalis plus intrauterine device (IUD)

            History

            associated with intrauterine device use; identified in patients with concurrent oral infection and use of copper-T 380A IUD

            Exam

            profuse vaginal discharge may be present

            1st investigation
            • washings from IUD for direct and stained smears plus DNA extraction/polymerase chain reaction:

              parasite present

            • concurrent testing for oral infection:

              parasite present

            Other investigations

              Inadequate hygiene

              History

              history of wiping from back to front, not changing tampons and pads in timely fashion, inadequate vaginal cleaning; pruritus; may have more frequent vaginal fungal infections

              Exam

              malodorous vaginal discharge, smegma

              1st investigation
              • none:

                diagnosis is clinical

              Other investigations

                Foreign body in adults

                History

                foul-smelling and/or bloody discharge

                Exam

                occasional irritation of labia and inner thighs; a longstanding foreign body can lead to extensive adhesion formation with near complete obstruction of the vagina inferior to the location of the foreign body

                1st investigation
                • pelvic examination:

                  visualisation or palpation of foreign body, may be full or partial obliteration of the vagina

                • abdominal/pelvic x-ray:

                  visualisation of foreign body

                  More
                Other investigations
                • transvaginal or transabdominal ultrasound:

                  visualisation of foreign body

                  More
                • MRI pelvis with oral and intravenous contrast:

                  visualisation of foreign body

                  More

                Combined contraceptive or hormonal vaginal ring-related

                History

                symptom onset with use of contraceptive ring

                Exam

                possible irritation at the site of ring pressure in the vagina

                1st investigation
                • diagnosis is clinical:

                  ring causes mostly localised irritation rather than vaginitis, but vaginitis should be ruled out with a wet mount

                Other investigations

                  Genitourinary syndrome of menopause

                  History

                  peri- or post-menopausal; itching, burning, discomfort, dyspareunia, yellowish malodorous vaginal discharge, or vaginal bleeding

                  Exam

                  atrophic epithelium appears pale, smooth, and shiny; inflammation with patchy erythema, petechiae and increased friability may be present

                  1st investigation
                  • none:

                    diagnosis is clinical (based on menopausal status)

                  Other investigations
                  • cytology of the vaginal epithelium:

                    atrophic cytological changes including increase in proportion of parabasal cells

                    More
                  • vaginal pH:

                    >5

                    More
                  • microscopy of vaginal swab for infectious cause:

                    negative for organisms that cause bacterial vaginosis, Trichomonas vaginalis, Candida species

                    More

                  Postpuerperal atrophic vaginitis; lochia

                  History

                  recent childbirth, lochia undergoes changes from red to white; itching, burning, discomfort, dyspareunia, yellowish malodorous vaginal discharge, or vaginal bleeding

                  Exam

                  reddish discharge in first few days, pinkish in next few days, and whitish from approximately 10th postnatal day onwards; findings of atrophic vaginitis subsequently: epithelium appears pale, smooth, and shiny, and inflammation with patchy erythema, petechiae, and increased friability may be present

                  1st investigation
                  • none:

                    diagnosis is clinical

                  Other investigations

                    Behcet's syndrome

                    History

                    known history of Behcet's syndrome; recurrent aphthous ulcers, genital ulcerations occasionally associated with vaginal discharge, and uveitis leading to blindness

                    Exam

                    recurrent genital ulcers in vulva and vagina, which are painful, and scarring; occasional vaginal discharge

                    1st investigation
                    • none:

                      diagnosis is clinical

                      More
                    Other investigations
                    • biopsy of genital ulcers:

                      vasculitis as well as lymphocytic and plasma cell invasion in the prickle cell layer of the epidermis

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                    Desquamative inflammatory vaginitis

                    History

                    chronic and exudative vaginitis associated with purulent and copious discharge; severe dyspareunia, minor vulvar symptoms such as irritation and/or pruritus

                    Exam

                    profuse and purulent vaginal discharge; spotted appearance or focal ecchymoses of vulva, vagina, and cervix

                    1st investigation
                    • wet mount microscopy of vaginal discharge:

                      polymorphonuclear infiltrate, basal and parabasal epithelial cells; absence of lactobacilli; no clue cells

                      More
                    Other investigations

                      Erosive lichen planus

                      History

                      chronic eruption mostly on flexor surfaces, mucous membranes, and vulvar skin; lesions usually extremely pruritic and sometimes painful

                      Exam

                      violaceous, shiny papules appearing mostly on flexor surfaces, mucous membranes, and vulvar skin; most lesions are located on the inner aspects of the vulva, especially on the labia minora and vestibule

                      1st investigation
                      • none:

                        diagnosis is clinical

                      Other investigations
                      • punch biopsy of vagina or vulva:

                        hyperkeratosis, degeneration of basal cell layer, infiltration of inflammatory cells and Rete pegs

                        More

                      Post-operative sling/mesh procedure

                      History

                      history intravaginal slingplasty or fistula; purulent and offensive vaginal discharge

                      Exam

                      malodorous vaginal discharge, visualised mesh erosion or fistula on speculum examination

                      1st investigation
                      • none:

                        diagnosis is clinical

                        More
                      Other investigations

                        Postradiation

                        History

                        history of pelvic irradiation

                        Exam

                        vaginal atrophy may also be present

                        1st investigation
                        • wet mount microscopy of vaginal discharge:

                          may help exclude other causes of vaginal discharge

                        Other investigations

                          Cervical cancer

                          History

                          some women can present with malodorous vaginal discharge indicating infection of a large, necrotic tumour; in late-stage cervical cancer, may have watery or foul-smelling vaginal discharge most likely from necrotic tissue

                          Exam

                          on speculum examination possible watery, foul-smelling discharge as well as pelvic fungating mass

                          1st investigation
                          • Pap smear:

                            epithelial cell abnormality

                          • biopsy of cervical mass:

                            positive for cervical cancer

                          Other investigations

                            Carcinoma of the fallopian tube

                            History

                            rare; only about 16% of patients present with the classic triad (hydrops tubae profluens) of vaginal discharge, colicky pelvic pain, and palpable pelvic mass, although data are very limited; vaginal bleeding; clear vaginal discharge is the most common symptom reported by patients

                            Exam

                            features characteristic of malignancy (e.g., weight loss, ascites)

                            1st investigation
                            • pelvic ultrasound:

                              neovascularisation on transvaginal colour Doppler or ascites suggest malignancy

                            Other investigations
                            • surgery (total abdominal hysterectomy and bilateral salpingo-oophorectomy, tumour debulking, full staging):

                              staging of disease

                              More
                            • CA-125:

                              increased with most patients with advanced tubal carcinoma

                              More
                            • CT of abdomen or pelvis with oral/intravenous contrast:

                              demonstrate metastatic disease

                            Pinworm infection

                            History

                            nocturnal vulvar and perianal pruritus

                            Exam

                            vulvar erythema, pinworms may be visible

                            1st investigation
                            • clear cellophane adhesive tape examined under the microscope:

                              pinworm eggs

                              More
                            Other investigations

                              Streptococcal vaginitis in children

                              History

                              current or recent symptomatic streptococcal pharyngitis; caused by transmission of respiratory flora from the nose and oral pharynx to the vulvar area

                              Exam

                              purulent discharge, beefy red-appearing vulva

                              1st investigation
                              • vaginal swab:

                                positive for Streptococcal pyogenes (group A streptococci)

                              Other investigations

                                Sexual abuse

                                History

                                foreign bodies in the vagina or rectum, genitourinary complaints, painful defecation or urination, vaginal discharge, bleeding or itching, grasp or rope marks, oral complaints, STIs, or possible pregnancy

                                Exam

                                most cases of sexual abuse of pre-pubertal girls have normal examination findings with a normal-appearing hymen; absence of all or parts of the hymen or a posterior hymenal tear represent signs of sexual abuse; profuse, yellow-to-green discharge may be a sign of infection; evaluation should be performed by a specialist in child sexual abuse

                                1st investigation
                                • cotton-tipped swabs for cultures:

                                  may be positive for Chlamydia trachomatis and Neisseria gonorrhoeae

                                  More
                                • nucleic acid amplification test:

                                  may be positive for Chlamydia trachomatis andNeisseria gonorrhoeae

                                  More
                                Other investigations
                                • wet mount microscopy of vaginal discharge:

                                  may be positive for Trichomonas vaginalis

                                  More
                                • viral culture of ulcerated lesions:

                                  may be positive for herpes simplex virus

                                • syphilis, HIV, hepatitis B and C serology:

                                  may be positive

                                  More

                                Transmitted maternal birth canal infection

                                History

                                vaginitis from Neisseria gonorrhoeae, Chlamydia, and Trichomonas can be found in neonates due to acquisition through an infected birth canal up to 1 year or more after birth; it is very important to rule out sexual abuse in these infants

                                Exam

                                profuse, yellow to green discharge

                                1st investigation
                                • cervical and vaginal nucleic acid amplification test of mother:

                                  may be positive for N gonorrhoeae, Chlamydia, and Trichomonas

                                • vaginal nucleic acid amplification test of infant:

                                  may be positive for N gonorrhoeae, Chlamydia, and Trichomonas

                                  More
                                Other investigations

                                  Prolapsing fibroid

                                  History

                                  history of fibroids, profuse discharge or bloody discharge

                                  Exam

                                  fibroid would be visible on physical examination or palpable within the cervix on bimanual examination

                                  1st investigation
                                  • ultrasound:

                                    sonographic appearance of uterine fibroid

                                  Other investigations

                                    Vaginal fistula

                                    History

                                    history of pelvic surgery, radiation, or Crohn's disease with continuous discharge consistent with urine or liquid stool

                                    Exam

                                    speculum examination, careful evaluation of the vaginal walls

                                    1st investigation
                                    • tampon test with placement of dye in bladder:

                                      positive if visualisation of dye in tampon

                                    Other investigations
                                    • retrograde pyelogram:

                                      shows leakage between ureter and vagina; sigmoidoscopy or anoscopy: fistula seen on probing

                                    • cystoscopy:

                                      fistula tract communicating with urinary bladder visualised

                                    • fistulogram:

                                      fistula tract visualised

                                    Lymphoma of genital tract

                                    History

                                    mass, malodorous discharge or vaginal bleeding, abdominal pain or fullness

                                    Exam

                                    a mass may be palpated on bimanual examination or a mass may be visualised on speculum examination

                                    1st investigation
                                    • pelvic ultrasound:

                                      confirms presence of mass

                                    • biopsy:

                                      positive for genital lymphoma

                                    Other investigations

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