A model of the pathogenesis is based on findings on macrophage and T-cell functions of affected patients.[28]Desnues B, Ihrig M, Raoult D, et al. Whipple's disease: a macrophage disease. Clin Vaccine Immunol. 2006 Feb;13(2):170-8.
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Macrophages of patients with Whipple disease display a persistently diminished ability to degrade intracellular organisms, and T. whipplei can prevent maturation of phagosomes.[29]Bai JC, Sen L, Diez R, et al. Impaired monocyte function in patients successfully treated for Whipple's disease. Acta Gastroenterol Latinoam. 1996;26(2):85-9.
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[30]Lukacs G, Dobi S, Szabo M. A case of Whipple's disease with repeated operations for ileus and complete cure. Acta Hepatogastroenterol (Stuttg). 1978 Jun;25(3):238-42.
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[31]Ghigo E, Capo C, Aurouze M, et al. Survival of Tropheryma whipplei, the agent of Whipple's disease, requires phagosome acidification. Infect Immun. 2002 Mar;70(3):1501-6.
http://iai.asm.org/cgi/content/full/70/3/1501
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Low production of interleukin (IL)-12 in monocytes, low serum concentrations of IL-12p40, and reduced expression of CD11b further indicate impaired macrophage functioning.[32]Marth T, Roux M, von Herbay A, et al. Persistent reduction of complement receptor 3 alpha-chain expressing mononuclear blood cells and transient inhibitory serum factors in Whipple's disease. Clin Immunol Immunopathol. 1994 Aug;72(2):217-26.
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[33]Ectors N, Geboes K, De Vos R, et al. Whipple's disease: a histological, immunocytochemical and electronmicroscopic study of the immune response in the small intestinal mucosa. Histopathology. 1992 Jul;21(1):1-12.
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[34]Kalt A, Schneider T, Ring S, et al. Decreased levels of interleukin-12p40 in the serum of patients with Whipple's disease. Int J Colorectal Dis. 2006 Mar;21(2):114-20.
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[35]Marth T, Neurath M, Cuccherini BA, et al. Defects of monocyte interleukin 12 production and humoral immunity in Whipple's disease. Gastroenterology. 1997 Aug;113(2):442-8.
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Intestinal macrophages reveal an alternatively activated phenotype.[36]Desnues B, Lepidi H, Raoult D, et al. Whipple disease: intestinal infiltrating cells exhibit a transcriptional pattern of M2/alternatively activated macrophages. J Infect Dis. 2005 Nov 1;192(9):1642-6.
http://jid.oxfordjournals.org/content/192/9/1642.full
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[37]Moos V, Schmidt C, Geelhaar A, et al. Impaired immune functions of monocytes and macrophages in Whipple's disease. Gastroenterology. 2010 Jan;138(1):210-20.
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Consequently, invading bacteria are ingested but not killed by intestinal macrophages.
In addition, the activity of type 1 helper T cells (Th1) is low in the periphery and the lamina propria of patients with Whipple disease.[38]Marth T, Kleen N, Stallmach A, et al. Dysregulated peripheral and mucosal Th1/Th2 response in Whipple's disease. Gastroenterology. 2002 Nov;123(5):1468-77.
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T whipplei-specific Th1 cells are reduced or absent in the periphery and the mucosa.[24]Moos V, Kunkel D, Marth T, et al. Reduced peripheral and mucosal Tropheryma whipplei-specific Th1 response in patients with Whipple's disease. J Immunol. 2006 Aug 1;177(3):2015-22.
http://www.jimmunol.org/cgi/content/full/177/3/2015
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By contrast, functional Th2 responses increase in peripheral and mucosal lymphocytes, in line with the observation that T whipplei replicates in macrophages deactivated by IL-4 and IL-10.[38]Marth T, Kleen N, Stallmach A, et al. Dysregulated peripheral and mucosal Th1/Th2 response in Whipple's disease. Gastroenterology. 2002 Nov;123(5):1468-77.
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[39]Schoedon G, Goldenberger D, Forrer R, et al. Deactivation of macrophages with interleukin-4 is the key to the isolation of Tropheryma whippelii. J Infect Dis. 1997 Sep;176(3):672-7.
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Impaired immunologic clearance of T whipplei results in massive accumulation of the bacteria in macrophages, mainly of the intestinal mucosa, which can be visualized by periodic acid-Schiff staining. Although the organ mainly affected is the GI tract, isolated CNS disease, infective endocarditis, and articular disease can occur. Subcutaneous nodules may develop in rare cases, and early manifestations may also be detected in the lung, liver, or muscle.[40]Friedmann AC, Perera GK, Jayaprakasam A, et al. Whipple's disease presenting with symmetrical panniculitis. Br J Dermatol. 2004 Oct;151(4):907-11.
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[41]Tarroch X, Vives P, Salas A, et al. Subcutaneous nodules in Whipple's disease. J Cutan Pathol. 2001 Aug;28(7):368-70.
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[42]Helliwell TR, Appleton RE, Mapstone NC, et al. Dermatomyositis and Whipple's disease. Neuromuscul Disord. 2000 Jan;10(1):46-51.
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[43]Kwee D, Fields JP, King LE Jr. Subcutaneous Whipple's disease. J Am Acad Dermatol. 1987 Jan;16(1 Pt 2):188-90.
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[44]Cho C, Linscheer WG, Hirschkorn MA, et al. Sarcoidlike granulomas as an early manifestation of Whipple's disease. Gastroenterology. 1984 Oct;87(4):941-7.
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[45]Viteri AL, Stinson JC, Barnes MC, et al. Rod-shaped organism in the liver of a patient with Whipple's disease. Dig Dis Sci. 1979 Jul;24(7):560-4.
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[46]Puget M, Iwaz J, Tristan A, et al. Whipple's disease with muscle impairment. Muscle Nerve. 2006 Dec;34(6):794-8.
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[47]Misbah SA, Stirzaker D, Ozols B, et al. Anonymous survey of blood donors by polymerase chain reaction for Tropheryma whippelii. QJM. 1999 Jan;92(1):61.
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