The diagnosis of narcolepsy is based on history followed by polysomnography (PSG) and a multiple sleep latency test (MSLT).
For full international classification of sleep disorders (ICSD) diagnostic criteria for narcolepsy type 1 and type 2, see Diagnostic criteria.[1]American Academy of Sleep Medicine. The AASM International classification of sleep disorders – third edition, text revision (ICSD-3-TR). Jun 2023 [internet publication].
https://aasm.org/clinical-resources/international-classification-sleep-disorders
History
Virtually all patients with narcolepsy have excessive daytime sleepiness (EDS). Patients may also report chronic fatigue or tiredness and memory problems. There may be a history of poor performance at work or of car accidents. The Epworth Sleepiness Scale (ESS) can be used for evaluating EDS.[73]Johns MW. A new method for measuring daytime sleepiness: the Epworth sleepiness scale. Sleep. 1991 Dec;14(6):540-5.
http://www.ncbi.nlm.nih.gov/pubmed/1798888?tool=bestpractice.com
[74]Johns MW. Reliability and factor analysis of the Epworth Sleepiness Scale. Sleep. 1992 Aug;15(4):376-81.
http://www.ncbi.nlm.nih.gov/pubmed/1519015?tool=bestpractice.com
[75]Chasens ER, Williams LL, Umlauf MG. Excessive sleepiness. In: Capezuti E, Zwicker D, Mezey M, et al, eds. Evidence-based geriatric nursing protocols for best practice. 3rd ed. New York, NY: Springer; 2009:459-76. People with untreated narcolepsy typically have ESS scores of ≥15.
People with narcolepsy are also prone to sleep attacks, which manifest as a high propensity to fall asleep in inappropriate situations several times a day.[76]Broughton R, Dunham W, Newman J, et al. Ambulatory 24 hour sleep-wake monitoring in narcolepsy-cataplexy compared to matched controls. Electroencephalogr Clin Neurophysiol. 1988 Dec;70(6):473-81.
http://www.ncbi.nlm.nih.gov/pubmed/2461281?tool=bestpractice.com
[77]Dantz B, Edgar DM, Dement WC. Circadian rhythms in narcolepsy: studies on a 90 minute day. Electroencephalogr Clin Neurophysiol. 1994 Jan;90(1):24-35.
http://www.ncbi.nlm.nih.gov/pubmed/7509271?tool=bestpractice.com
This can take place during monotonous, sedentary activity; post-prandially; or when fully involved in a task. Episodes may last a few minutes to longer than 1 hour.[78]Guilleminault C, Gelb M. Clinical aspects and features of cataplexy. Adv Neurol. 1995;67:65-77.
http://www.ncbi.nlm.nih.gov/pubmed/8848983?tool=bestpractice.com
[79]Anic-Labat S, Guilleminault C, Kraemer HC, et al. Validation of a cataplexy questionnaire in 983 sleep-disorders patients. Sleep. 1999 Feb 1;22(1):77-87.
http://www.ncbi.nlm.nih.gov/pubmed/9989368?tool=bestpractice.com
Patients may report low physical or mental energy, a frequent correlate of sleep disorders; poor memory and concentration, which may show progressive deterioration, frequently being attributed to ageing; reduced work capabilities including inattention and inability to sustain repetitive tasks; and car accidents due to sleepiness and overt dozing.
Most (but not all) people with narcolepsy type 1 have cataplexy (generalised muscle weakness leading to partial or complete collapse), which can be present at the time of the diagnosis or appear within 3 to 5 years after the perceived onset of sleepiness.[16]Okun ML, Lin L, Pelin Z, et al. Clinical aspects of narcolepsy-cataplexy across ethnic groups. Sleep. 2002 Feb 1;25(1):27-35.
http://www.ncbi.nlm.nih.gov/pubmed/11833858?tool=bestpractice.com
Narcolepsy type 2 is not associated with cataplexy.[1]American Academy of Sleep Medicine. The AASM International classification of sleep disorders – third edition, text revision (ICSD-3-TR). Jun 2023 [internet publication].
https://aasm.org/clinical-resources/international-classification-sleep-disorders
Cataplexy is most frequently triggered by emotional stimuli such as laughter, excitement, or anger.[5]Guilleminault C, Fromherz S. Narcolepsy: diagnosis and management. In: Kryger MH, Roth T, Dement WC, eds. Principles and practice of sleep medicine. 4th ed. Philadelphia, PA: Elsevier Saunders; 2005. Speech may be impaired, vision may be blurred, and respiration may become irregular during an attack. A complete loss of muscle tone can lead to collapse and the risk of head trauma or bone fractures. Status cataplecticus is a rare manifestation of cataplexy, characterised by prolonged cataplexy that lasts for hours.[5]Guilleminault C, Fromherz S. Narcolepsy: diagnosis and management. In: Kryger MH, Roth T, Dement WC, eds. Principles and practice of sleep medicine. 4th ed. Philadelphia, PA: Elsevier Saunders; 2005. Patients often fall asleep after a cataplectic attack. People often report hypnagogic hallucinations (visual or auditory perceptions on falling asleep) or hypnopompic hallucinations (visual or auditory perceptions on awakening).
Many people with narcolepsy also report sleep paralysis, which may be accompanied by hypnopompic hallucinations and a feeling of suffocation. It is sometimes difficult to differentiate these from anxiety attacks. In anxiety, inexplicable fear is often the initial event, followed by inability to move.
There may be a history of disturbed night-time sleep, or of other sleep disorders, such as obstructive sleep apnoea (OSA), periodic limb movement disorder of sleep, and rapid eye movement (REM) sleep behaviour disorder, which are more common with narcolepsy.[1]American Academy of Sleep Medicine. The AASM International classification of sleep disorders – third edition, text revision (ICSD-3-TR). Jun 2023 [internet publication].
https://aasm.org/clinical-resources/international-classification-sleep-disorders
[80]Howell M, Avidan AY, Foldvary-Schaefer N, et al. Management of REM sleep behavior disorder: an American Academy of Sleep Medicine clinical practice guideline. J Clin Sleep Med. 2023 Apr 1;19(4):759-68.
https://www.doi.org/10.5664/jcsm.10424
http://www.ncbi.nlm.nih.gov/pubmed/36515157?tool=bestpractice.com
It is important to diagnose comorbid sleep disorders, as they may contribute to EDS, and therapy differs.[5]Guilleminault C, Fromherz S. Narcolepsy: diagnosis and management. In: Kryger MH, Roth T, Dement WC, eds. Principles and practice of sleep medicine. 4th ed. Philadelphia, PA: Elsevier Saunders; 2005. Patients with narcolepsy have frequent sleep-onset REM sleep periods or REM intrusions into wakefulness. This may be elicited from the history as dreams during transitions from sleep to waking and vice versa, dreams during short daytime naps, and difficulty in differentiating such dream episodes from reality.
There may be a family history of sleep disorders, or a history of central nervous system trauma or infection.
Clinical examination
Usually normal, except in patients with narcolepsy due to a medical condition, in which case clinical signs of the underlying condition may be elicited (e.g., obesity in Prader-Willi syndrome, hepatomegaly in Niemann-Pick disease, and paresis in multiple sclerosis).
Diagnostic tests
Although narcolepsy (with or without cataplexy) is sometimes diagnosed clinically, a definitive diagnosis requires further diagnostic testing.[1]American Academy of Sleep Medicine. The AASM International classification of sleep disorders – third edition, text revision (ICSD-3-TR). Jun 2023 [internet publication].
https://aasm.org/clinical-resources/international-classification-sleep-disorders
[81]Golden EC, Lipford MC. Narcolepsy: Diagnosis and management. Cleve Clin J Med. 2018 Dec;85(12):959-69.
https://www.doi.org/10.3949/ccjm.85a.17086
http://www.ncbi.nlm.nih.gov/pubmed/30526757?tool=bestpractice.com
[82]American Academy of Sleep Medicine. The AASM manual for scoring of sleep and associated events. Feb 2023 [internet publication].
https://aasm.org/clinical-resources/scoring-manual
PSG and MSLT
The first investigation in people with suspected narcolepsy is an overnight PSG, which is generally followed by MSLT.[83]Littner M, Johnson SF, McCall WV, et al. Practice parameters for the treatment of narcolepsy: an update for 2000. Sleep. 2001 Jun 15;24(4):451-66.
http://www.ncbi.nlm.nih.gov/pubmed/11403530?tool=bestpractice.com
It is strongly recommended that the MSLT be preceded by at least 1 week of actigraphic recording with a sleep log or diary to clarify if the results could be confounded by insufficient sleep, shift work, or another circadian sleep disorder.[1]American Academy of Sleep Medicine. The AASM International classification of sleep disorders – third edition, text revision (ICSD-3-TR). Jun 2023 [internet publication].
https://aasm.org/clinical-resources/international-classification-sleep-disorders
PSG ascertains sleep quality before the MSLT and can identify alternative or co-existing causes of EDS, such as OSA, periodic limb movement disorder of sleep, or REM sleep behaviour disorder.[1]American Academy of Sleep Medicine. The AASM International classification of sleep disorders – third edition, text revision (ICSD-3-TR). Jun 2023 [internet publication].
https://aasm.org/clinical-resources/international-classification-sleep-disorders
[80]Howell M, Avidan AY, Foldvary-Schaefer N, et al. Management of REM sleep behavior disorder: an American Academy of Sleep Medicine clinical practice guideline. J Clin Sleep Med. 2023 Apr 1;19(4):759-68.
https://www.doi.org/10.5664/jcsm.10424
http://www.ncbi.nlm.nih.gov/pubmed/36515157?tool=bestpractice.com
[82]American Academy of Sleep Medicine. The AASM manual for scoring of sleep and associated events. Feb 2023 [internet publication].
https://aasm.org/clinical-resources/scoring-manual
The PSG may be normal, but often shows snoring, frequent awakenings, mildly reduced sleep efficiency, reduced sleep latency (<10 minutes), periods of REM sleep within the first 15 minutes of sleep, and REM fragmentation by multiple spontaneous arousals.
An MSLT is performed only if the PSG performed the night before shows >6 hours of total sleep time.[84]Krahn LE, Arand DL, Avidan AY, et al. Recommended protocols for the Multiple Sleep Latency Test and Maintenance of Wakefulness Test in adults: guidance from the American Academy of Sleep Medicine. J Clin Sleep Med. 2021 Dec 1;17(12):2489-98.
https://www.doi.org/10.5664/jcsm.9620
http://www.ncbi.nlm.nih.gov/pubmed/34423768?tool=bestpractice.com
In general, the naps of patients with narcolepsy contain periods of REM sleep. A mean sleep latency of ≤8 minutes plus 2 or more sleep-onset REM periods (SOREMPs) is diagnostic for narcolepsy.[1]American Academy of Sleep Medicine. The AASM International classification of sleep disorders – third edition, text revision (ICSD-3-TR). Jun 2023 [internet publication].
https://aasm.org/clinical-resources/international-classification-sleep-disorders
[84]Krahn LE, Arand DL, Avidan AY, et al. Recommended protocols for the Multiple Sleep Latency Test and Maintenance of Wakefulness Test in adults: guidance from the American Academy of Sleep Medicine. J Clin Sleep Med. 2021 Dec 1;17(12):2489-98.
https://www.doi.org/10.5664/jcsm.9620
http://www.ncbi.nlm.nih.gov/pubmed/34423768?tool=bestpractice.com
By contrast, healthy subjects usually fall asleep in 10 to 15 minutes.[85]Roehrs T, Roth T. Multiple sleep latency test: technical aspects and normal values. J Clin Neurophysiol. 1992 Jan;9(1):63-7.
http://www.ncbi.nlm.nih.gov/pubmed/1552009?tool=bestpractice.com
However, there are a number of weaknesses in the ability of the MSLT to definitively diagnose narcolepsy, and other factors, such as sex, age, shift work, and medicine use, may also affect SOREMPs.[86]Mignot E, Lin L, Finn L, et al. Correlates of sleep-onset REM periods during the multiple sleep latency test in community adults. Brain. 2006 Jun;129(Pt 6):1609-23.
http://brain.oxfordjournals.org/content/129/6/1609.full
http://www.ncbi.nlm.nih.gov/pubmed/16597649?tool=bestpractice.com
[87]Dauvilliers Y, Gosselin A, Paquet J, et al. Effect of age on MSLT results in patients with narcolepsy-cataplexy. Neurology. 2004 Jan 13;62(1):46-50.
http://www.ncbi.nlm.nih.gov/pubmed/14718696?tool=bestpractice.com
Therefore, if there is a strong suspicion of narcolepsy, repetition of the MSLT is required.[86]Mignot E, Lin L, Finn L, et al. Correlates of sleep-onset REM periods during the multiple sleep latency test in community adults. Brain. 2006 Jun;129(Pt 6):1609-23.
http://brain.oxfordjournals.org/content/129/6/1609.full
http://www.ncbi.nlm.nih.gov/pubmed/16597649?tool=bestpractice.com
The MSLT is not appropriate for diagnosing narcolepsy in shift workers unless they have resumed a normal circadian pattern.[1]American Academy of Sleep Medicine. The AASM International classification of sleep disorders – third edition, text revision (ICSD-3-TR). Jun 2023 [internet publication].
https://aasm.org/clinical-resources/international-classification-sleep-disorders
Other tests
Human leukocyte antigen (HLA) typing may be useful in certain situations, because the majority of the patients with narcolepsy are HLA-DQB1*0602 positive, but this is not diagnostic for narcolepsy. HLA typing may be considered if lumbar puncture is being contemplated to measure cerebrospinal fluid (CSF) hypocretin-1 levels, since any patient who is HLA-negative is very likely to have normal hypocretin-1 levels.[1]American Academy of Sleep Medicine. The AASM International classification of sleep disorders – third edition, text revision (ICSD-3-TR). Jun 2023 [internet publication].
https://aasm.org/clinical-resources/international-classification-sleep-disorders
Low CSF hypocretin-1 levels (110 picograms/mL or less than one third of mean values obtained in normal subjects with the same standardised assay) are a diagnostic marker for narcolepsy type 1 in patients with EDS.[1]American Academy of Sleep Medicine. The AASM International classification of sleep disorders – third edition, text revision (ICSD-3-TR). Jun 2023 [internet publication].
https://aasm.org/clinical-resources/international-classification-sleep-disorders
However, this requires a lumbar puncture, which is painful for patients, and is usually indicated only if MSLT results are uninterpretable or equivocal due to poor sleep efficiency (e.g., concurrent sleep disorders) or MSLT is not suitable (e.g., shift worker, or an inability to discontinue psychoactive drugs with REM sleep suppressive action.[1]American Academy of Sleep Medicine. The AASM International classification of sleep disorders – third edition, text revision (ICSD-3-TR). Jun 2023 [internet publication].
https://aasm.org/clinical-resources/international-classification-sleep-disorders
[88]Mignot E, Lammers GJ, Ripley B, et al. The role of cerebrospinal fluid hypocretin measurement in the diagnosis of narcolepsy and other hypersomnias. Arch Neurol. 2002 Oct;59(10):1553-62.
http://archneur.jamanetwork.com/article.aspx?articleid=782942
http://www.ncbi.nlm.nih.gov/pubmed/12374492?tool=bestpractice.com
[89]Overeem S, Scammell TE, Lammers GJ. Hypocretin/orexin and sleep: implications for the pathophysiology and diagnosis of narcolepsy. Curr Opin Neurol. 2002 Dec;15(6):739-45.
http://www.ncbi.nlm.nih.gov/pubmed/12447114?tool=bestpractice.com
CSF hypocretin levels are not affected by sleep deprivation, circadian disturbance, disordered breathing, or medicine use or discontinuation, but may be lowered in some seriously ill patients.[1]American Academy of Sleep Medicine. The AASM International classification of sleep disorders – third edition, text revision (ICSD-3-TR). Jun 2023 [internet publication].
https://aasm.org/clinical-resources/international-classification-sleep-disorders
The maintenance of wakefulness test (MWT) may be indicated when the inability to remain awake constitutes a safety risk.[84]Krahn LE, Arand DL, Avidan AY, et al. Recommended protocols for the Multiple Sleep Latency Test and Maintenance of Wakefulness Test in adults: guidance from the American Academy of Sleep Medicine. J Clin Sleep Med. 2021 Dec 1;17(12):2489-98.
https://www.doi.org/10.5664/jcsm.9620
http://www.ncbi.nlm.nih.gov/pubmed/34423768?tool=bestpractice.com
Actigraphy can be used for monitoring the nocturnal arousals/limb movements or to document the circadian patterns.[90]Smith MT, McCrae CS, Cheung J, et al. Use of actigraphy for the evaluation of sleep disorders and circadian rhythm sleep-wake disorders: an American Academy of Sleep Medicine clinical practice guideline. J Clin Sleep Med. 2018 Jul 15;14(7):1231-7.
https://www.ncbi.nlm.nih.gov/labs/pmc/articles/PMC6040807
http://www.ncbi.nlm.nih.gov/pubmed/29991437?tool=bestpractice.com