Investigations

1st investigations to order

core temperature measurement

Test
Result
Test

Measure and monitor the patient’s core temperature.

  • Check your local protocols. If the patient has severe hypothermia, common practice in hospital emergency departments in the UK is to use an oesophageal probe (a probe positioned in the lower third of the oesophagus) if the patient has a secured and protected airway (i.e., tracheal tube or a supraglottic device with an oesophageal channel in place), or to measure rectal temperature.

    • Bear in mind, however, that the 2021 European Resuscitation Council (ERC) guideline has de-emphasised measurement of rectal temperature (or bladder temperature, which may be used if the patient requires a bladder catheter) for patients with severe hypothermia because temperature at these sites lags behind core temperature.[24]

    • Never measure rectal temperature if the patient is in a cold environment because this method requires the patient to be further exposed, which will increase heat loss and potentially worsen the hypothermia.[4] 

  • Where feasible (usually in hospital) the 2021 ERC and 2019 Wilderness Medical Society guidelines recommend:

    • Preferably: an oesophageal probe.[24][4] An oesophageal probe correlates well with the temperature of the pulmonary artery and is the preferred method when available.[3][4] 

      OR

    • Alternatively: a low-reading tympanic membrane thermistor-based thermometer (where the thermistor touches the tympanic membrane) if the patient is spontaneously breathing.[24][4] Thermistor-based thermometers may not be widely available in some countries, including the UK.

  • Do not use a standard clinical thermometer to measure core temperature. This may be inadequate because it will not measure temperatures below 34.4°C (94°F). Conventional mercury thermometers are no longer recommended, owing to the risk of breakage and poisoning.

  • If the preferred methods for measuring core temperature are not available (e.g., in a pre-hospital setting), suspect hypothermia from the history and setting, and assess whether the patient’s trunk feels cold.[24]

Practical tip

Cold water or snow in the patient’s ear canal can lead to localised cooling of the tympanic membrane, which may result in a falsely low core temperature reading.

Result

<35°C (<95°F)

12-lead ECG

Test
Result
Test

Monitor the ECG continuously.

  • This is essential for detecting arrhythmias, which may be fatal. Where possible, ECG monitoring should also be used to detect cardiac arrest.[4][24]

  • Arrhythmias can occur at any stage of hypothermia, and also during re-warming. Initially, in mild hypothermia, the ECG may show tachycardia. In more severe cases of hypothermia, the ECG may show progressive sinus bradycardia, atrial or ventricular fibrillation, junctional rhythms, ST segment changes, T-wave inversion, prolongation of the QT interval, and eventually asystole.[5] With the exception of ventricular fibrillation, these changes are likely to improve without treatment as the patient’s core temperature increases.[4][24]

  • J waves (or Osborn waves) occur in most, but not all, patients.[27] However, they do not correlate well with temperature.[28]

[Figure caption and citation for the preceding image starts]: A 12 lead ECG obtained from a hypothermic patient; note Osborn waves (arrows), which have an extra deflection at the end of the QRS complexAydin M, Gursurer M, Bayraktaroglu T, et al. Tex Heart Inst J. 2005;32(1):105 [Citation ends].A 12 lead ECG obtained from a hypothermic patient; note Osborn waves (arrows), which have an extra deflection at the end of the QRS complex

Result

sinus bradycardia; atrial fibrillation; J wave or Osborn wave; ST elevation or depression; T wave inversion; prolonged PR, QT, and QTc interval; broad QRS complexes

blood glucose

Test
Result
Test

Bear in mind that glucose levels may be normal, high (owing to increased secretion of stress hormones - cortisol, growth hormones, and catecholamines - and reduced insulin secretion, together with increased peripheral resistance to insulin), or low (owing to cold-induced inhibition of hepatic glucose production).

  • Monitor blood glucose even after the patient is normoglycaemic because rebound hypoglycaemia may develop when normal insulin production resumes.

  • Treat hypoglycaemia promptly. Hypoglycaemia can stop shivering (because the central control of shivering is dependent on glucose), leading to subsequent heat loss.[29]

Result

may be elevated, often normal, sometimes low

blood gas

Test
Result
Test

Note that blood gases may show respiratory alkalosis, metabolic acidosis, or a mixture of both.

  • As core temperature decreases, respiration is depressed, resulting in hypoxaemia and hypercapnia.

  • A combined respiratory and metabolic acidosis occurs as a result of hypoventilation, retention of carbon dioxide, decreased bicarbonate, impaired hepatic metabolism of organic acid production (owing to impaired hepatic perfusion), and increased lactic acid production. It is important to note that blood pH rises by 0.015 for every 1°C (1.8°F) drop in body temperature.

In general, use blood gas results without adjustment for temperature to guide treatment decisions.[30]

  • Be aware that there is debate in the literature about how to interpret blood gases in patients with hypothermia.[3][30] This is a complex issue, because blood gas analysers warm the blood sample to 37℃ (98.6℉) - higher than the patient’s temperature if they are hypothermic - and pH, PO2 and PCO2 all vary with temperature.[3][30] Therefore, interpretation of uncorrected blood gas results is a widely used approach because clinicians are more familiar with this.[30]

In practice, perform a venous rather than an arterial blood gas; a venous blood gas is associated with less risk than an arterial blood gas and gives adequate results for most patients.

Monitor blood gases to ensure resolution of hypoxia and normalisation of pH.

Result

respiratory alkalosis, metabolic acidosis, or a mixture of both

  • respiratory acidosis is suggested by pH <7.35 and PCO2 >40 mmHg

  • metabolic acidosis is suggested by pH <7.35, bicarbonate ≤24 mEq/L, and a normal PCO2, although it may be low with compensation

  • PaO2 may be low with severe hypothermia, and/or if there are pulmonary infiltrates or oedema 

serum urea, electrolytes, and creatinine

Test
Result
Test

Hypokalaemia may occur as a result of the hypothermia or the associated treatment. Hyperkalaemia may occur during re-warming.

  • For a patient in cardiac arrest, hyperkalaemia can also indicate that hypoxia preceded hypothermia (e.g., if the patient was found in an avalanche).[4] 

  • Hyperkalaemia is part of the HOPE (Hypothermia Outcome Prediction after ECLS re-warming for hypothermic arrested patients) score for prognostication of successful re-warming.[4][24] Initial serum potassium >12 mmol/L (12 mEq/L) is associated with irreversible death if the patient is in cardiac arrest.[3]

Renal function may be impaired due to dehydration, cold exposure, or rhabdomyolysis.

Result

hypokalaemia, hyperkalaemia

FBC

Test
Result
Test

Haemoglobin and haematocrit may be elevated due to haemoconcentration. Platelets and WBCs are abnormally low due to sequestration in the spleen.

Result

elevated haemoglobin and haematocrit, low WBC and platelet counts

clotting screen

Test
Result
Test

Prothrombin time (PT) and PTT are prolonged due to inhibition of enzymatic activity in both the intrinsic and the extrinsic coagulation cascade. The cause of coagulopathy is unknown.[31]

Result

elevated PT and PTT

chest x-ray

Test
Result
Test

A chest x-ray is particularly important if the patient has an altered level of consciousness.

  • It may show pulmonary oedema or infiltrates.

  • If the patient has been immersed in water, it may show inhaled foreign bodies, such as false teeth or debris from the water, which will need to be removed. See our topic Foreign body aspiration.

Result

may be normal or may show pulmonary infiltrates, oedema, or foreign bodies

Investigations to consider

serum creatine kinase

Test
Result
Test

Order if the patient has not been immersed in water and therefore may have been lying on the ground for a long time, to check for rhabdomyolysis. See our topic Rhabdomyolysis.

Result

>5 times the upper limit of normal indicates rhabdomyolysis

myoglobin levels

Test
Result
Test

Order if the patient has not been immersed in water and therefore may have been lying on the ground for a long time, to check for rhabdomyolysis. See our topic Rhabdomyolysis.

Result

increased levels in blood and/or urine is an indicator of rhabdomyolysis

end-tidal CO₂

Test
Result
Test

Use to detect cardiac arrest, where possible, in the absence of vital signs.[24][4]

Result

low or absent in cardiac arrest

ultrasound

Test
Result
Test

Use to detect cardiac arrest, where possible, in the absence of vital signs.[24][4]

Result

ventricular fibrillation or absence of cardiac activity in cardiac arrest

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