Investigations

1st investigations to order

chest x-ray

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Result
Test

Standard posteroanterior and lateral CXR is an inexpensive and simple initial study to evaluate cough, chest pain, and/or haemoptysis.

Result

central or peripheral mass, hilar lymphadenopathy, superior mediastinal lymphadenopathy, pleural effusion

CT chest, liver, and adrenal glands

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Result
Test

A new abnormality on chest x-ray should be further assessed with CT. A chest CT should also be obtained in patients, especially smokers, with concerning symptoms and a normal chest x-ray. Intravenous contrast is helpful to distinguish lymph nodes from vessels, especially in the hilum.

Limited disease: involvement restricted to ipsilateral hemithorax within a single radiation port. Extensive disease: presence of contralateral pulmonary or other distant metastasis.[5][32]​​​

After treatment, chest CT (or chest x-ray) can be obtained to evaluate response to treatment and monitor for disease recurrence.

Result

massive lymphadenopathy and direct mediastinal invasion are common features of SCLC; determines extent of disease

Investigations to consider

bronchoscopy

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Result
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Bronchoscopy is performed when CT abnormalities (i.e., a mass or adenopathy) are accessible to the bronchoscope. It is also used to assess new and/or unexplained pulmonary symptoms (i.e., haemoptysis, wheezing, cough). Flexible bronchoscopy requires conscious sedation. During the procedure, the tracheobronchial tree is carefully examined. Endobronchial tumours can be biopsied. Washings, brushings, and bronchoalveolar lavage are performed. Suspicious parenchymal lesions and mediastinal lymph nodes that are accessible can also be biopsied.[31]

Endobronchial masses can be biopsied with forceps. Endobronchial brushings, washings, and alveolar lavage increase the diagnostic yield. Trans-bronchial needle aspiration of accessible parenchymal lesions and mediastinal lymph nodes is possible.

Overall, the sensitivity for centrally located lesions is high (about 90%).[31] The sensitivity for peripheral lesions is lower and depends on number of biopsies taken, size of mass, and proximity to the bronchial tree. In general, endobronchial biopsy is more sensitive than brushings or washings.

Detection of small peripheral lesions (<2 cm) is improved by use of endobronchial ultrasound.[37]

Bronchoscopy can be repeated after definitive treatment to assess for recurrent disease.

Result

endobronchial lesions

biopsy

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Result
Test

Pathological confirmation of malignancy is the only widely accepted method to diagnose lung cancer. Tissue may be sampled with bronchoscopy if lesions are central, but specimen adequacy for diagnosis is variable. Transbronchial biopsy or endobronchial biopsy, brushings, and lavage are all used to obtain specimens during bronchoscopy. CT-guided needle biopsy, where feasible, allows for core biopsies to obtain sufficient material. If there are suspected metastatic lesions, those are preferred sites for diagnostic biopsy if feasible to confirm metastatic disease. Method of biopsy for metastatic sites depends on the anatomical location and least invasive approach.[32]

Result

malignant cells, high nuclear to cytoplasmic ratio, nuclear fragmentation often present

thoracentesis

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Result
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Thoracentesis involves placing a needle between the ribs and into the chest to sample fluid that has accumulated in the pleural space. Ultrasound is helpful in directing thoracentesis of small pleural effusions.[5]


Pleural aspiration animated demonstration
Pleural aspiration animated demonstration

Video demonstrating how to perform a pleural aspiration


Result

malignant cells within the pleural fluid

thoracoscopy

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Result
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May be considered in patients with SCLC and pleural effusion, if thoracentesis is inconclusive, to determine stage.[5]​​

Result

pleural involvement

MRI or CT of brain

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Result
Test

All patients with SCLC should be evaluated for brain metastases, ideally with an MRI.[5][32]​​​

Result

brain metastases appear as enhancing parenchymal masses with surrounding oedema

bone scan

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Result
Test

May be used to identify metastases if PET/CT is not available.[5]​​

Result

skeletal metastases

mediastinoscopy

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Result
Test

Indicated to determine nodal status in patients with a solitary pulmonary mass without radiographic evidence of lymphadenopathy, as these patients may be candidates for surgery. Can also be used for initial diagnosis.[5]

Result

node involvement

positron emission tomography (PET)

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Result
Test

PET is complementary to CT, facilitating accurate assessment of the extent of local, regional, and distant disease.[5][32]

Fluorodeoxyglucose (FDG)-PET/CT scan is superior to PET alone.[5]​​​​

PET-CT can also be performed prior to tissue sampling in order to identify possible metastatic lesion(s) that can be biopsied, determining stage and confirming pathological diagnosis in the same procedure.

Result

further evaluates location and extent of primary tumour; evaluates for distant metastases

bone marrow aspirate and biopsy

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Result
Test

Indicated for staging in patients who present with nucleated red cells on peripheral smear, or unexplained anaemia or thrombocytopenia, and no other evidence of metastatic disease.[5][32]​​​

Result

bone marrow invasion by cancer cells

FBC

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Result
Test

Baseline blood counts are necessary before treatment is initiated or invasive procedures are performed.[5][32]

Chemotherapy, and to a lesser degree radiotherapy, can decrease haematopoiesis, necessitating baseline and periodic analysis of blood counts.

Result

usually normal; however, may show anaemia

LFTs

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Result
Test

Hepatic metastases may cause elevated LFTs.

Elevated alkaline phosphatase level may indicate bony metastases.[5][32]

Result

normal or elevated

serum sodium

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Result
Test

Hyponatraemia occurs in syndrome of inappropriate antidiuretic hormone.[5][32]

Result

lowered

renal function

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Result
Test

Baseline assessment is recommended before initiation of treatment.

Some chemotherapy agents, cisplatin in particular, can affect electrolytes and kidney function.[5][32]

Result

usually normal

lung function tests

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Result
Test

FEV₁ and diffusion capacity of lung for carbon monoxide (DLCO) should be performed on all patients expected to receive radiation.[32][33][34]​​​​

Result

spirometry and lung volumes

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