Ranges of ΔCq values and inferred volumes of EB dilutions transferred
| Category (n) | Mean ΔCq (SD) | ΔCq range | Mean µL transferred (inferred from±2xΔCq SD range)(inferred from observed ΔCq range) | |
| All (49) | 10.0 (1.6) | 6.7–13.3 | 9.8 (1.1–89.7)(1.0–96.2) | |
| Participant 1 (17) | 8.8 (0.9) | 6.7–9.8 | 22.4 (6.4—78.1)(11.2–96.2) | p<0.0001* Participant 1 vs participant 2: p<0.01† Participant 1 vs participant 3: p<0.01† Participant 2 vs participant 3: not significant† |
| Participant 2 (16) | 10.5 (1.2) | 8.6–13.0 | 7.1 (1.4–32.6)(1.2–25.8) | |
| Participant 3 (16) | 10.9 (1.6) | 7.7–13.3 | 5.2 (0.6–48.1)(1.0–48.1) | |
| Dipping (23) | 10.6 (1.7) | 7.4–13.3 | 6.4 (0.6–68.0)(1.0–59.2) | p=0.015‡ |
| Pouring (26) | 9.5 (1.3) | 6.7–12.4 | 13.8 (2.3–83.7)(1.0–96.2) | |
| E_Aus56 (17) | 9.7 (1.6) | 6.7–12.2 | 12.0 (1.3–110.5)(2.1–96.2) | Not significant; p=0.41* |
| F_Aus51 (15) | 10.0 (1.6) | 7.4–13.3 | 9.8 (1.1–89.8)(1.0–59.2) | |
| B_Aus45 (17) | 10.4 (1.5) | 8.4–13.0 | 7.4 (0.9–59.2)(1.2–29.6) | |
| Undiluted (18) | 9.4 (1.7) | 6.7–12 | 14.8 (1.4–156.3)(2.4–96.2) | p=0.004* 100 vs 10−2: p<0.01† 100 vs 10−4: not significant† 102 vs 10−4: p<0.05† |
| 10−2 diluted (18) | 11.0 (1.5) | 9.0–13.3 | 4.8 (0.6–39.1)(1.0–19.5) | |
| 10−4 diluted (13) | 9.5 (1.0) | 7.7–11.1 | 13.8 (3.5–55.2)(4.6–48.1) |
The 49 data points were collated according to different criteria to reveal possible correlates. This encompasses data only from specimens that yielded a positive C. trachomatis test with a Cq <40.
*One way analysis of variance.
†Tukey HSD.
‡t-test (two tailed).