Table 1

Luminex data for mean cytokine and chemokine levels (pg/ml) in ankles (regular font) and serum (italicised font) for studies of genetic (IL1R1 knockout mice) or pharmacological (mIL1 Trap) depletion of IL1 signalling

Protein	IL1R1 Knockout Study				mIL1 Trap Study
	PBS		MSU		PBS		MSU
	WT	KO	WT	KO	Vehicle	Trap	Vehicle	Trap
IL1β†	<3.2	<3.2	39.6	8.9†	5.5	5.9	30.0	10.0†
IL6‡	<3.2	<3.2	150.1	21.0†	43.0	48.6	198.1	94.7†
MCP-1‡	10.9	7.9	638.3	293.7†	7.1	<3.2	823.4	748.2
MIP-1α‡	<3.2	<3.2	494.4	113.7‡	18.0	43.8	351.2	216.3‡
KC‡	4.1	<3.2	608.8	90.0‡	5.9	<3.2	955.3	579.4†
TNFα‡	<3.2	<3.2	6.0	3.8†	<3.2	<3.2	10.4	4.6*
G-CSF#	<3.2	<3.2	261.7	4.8‡	<3.2	<3.2	232.9	16.1‡
IL6*	<3.2	6.7	33.2	5.9†	5.0	10.0	36.0	27.6
KC/†*	26.6	32.3	80.1	33.7*	34.9	30.1	66.8	82.3
G-CSF	52.9	33.6	334.2	51.8‡	31.1	26.2	87.0	35.3‡

Only proteins significantly upregulated by MSU crystals are shown (level of significance indicated after protein name). Asterisks after data values indicate that the value is significantly different from controls (WT or vehicle), *p<0.05, †p<0.01, ‡p<0.001.
G-CSF, granulocyte-colony stimulating factor; IL1R1, interleukin 1 receptor type 1; KC, keratinocyte chemoattractant; KO, knockout; MCP-1, monocyte chemotactic protein-1; mIL1, murine interleukin 1; MIP-1, macrophage inhibitory protein-1; MSU, monosodium urate; PBS, phosphate-buffered saline; TNF, tumour necrosis factor; WT, wild-type.