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Inflammation and inflammatory bowel disease
Upregulation of Reg 1α and GW112 in the epithelium of inflamed colonic mucosa
  1. S Shinozakia,
  2. T Nakamuraa,
  3. M Iimuraa,c,
  4. Y Katob,
  5. B Iizukaa,
  6. M Kobayashib,
  7. N Hayashia
  1. aInstitute of Gastroenterology, Tokyo Women's Medical University, Tokyo, Japan, bDepartment of Pathology, Tokyo Women's Medical University, Tokyo, Japan, cClinical Research Institute, National Yokohama Hospital, Yokohama, Japan
  1. Dr T Nakamura, Institute of Gastroenterology, Tokyo Women's Medical University, 8-1, Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan. if9t-nkmr{at}asahi-net.or.jp

Abstract

BACKGROUND AND AIMS Colonic epithelium is involved in the regulation of intestinal function and mucosal immune responses, and its function is altered in inflammatory bowel disease (IBD). However, a comprehensive analysis of the genetic alterations in inflamed colonic epithelium is not available at present. The aim of our study was to detect genes that are preferentially expressed in inflamed colonic epithelia and clarify the biochemical responses of epithelial cells in inflamed colonic mucosa.

METHODS cDNA representation difference analysis was used to identify candidate genes selectively expressed in inflamed colonic epithelia. Selective expression of these genes in the epithelium of inflamed colonic mucosa, including IBD and non-IBD tissues, was examined by real time polymerase chain reaction and in situ hybridisation. The effect of cell confluence and inflammatory mediators on Reg 1α gene expression was examined using a colon cancer cell line (HT29).

RESULTS We identified seven candidate genes that were presumed to be upregulated in the inflamed colonic epithelium. Of these, Reg 1α and GW112 were the dominant species and expression of these genes was confined to the crypt epithelium. In vitro studies using a colonic epithelial cell line suggested that cell confluence regulates Reg 1α gene expression.

CONCLUSIONS Selective expression of Reg 1α and GW112 genes in the crypt epithelium of inflamed colonic mucosa suggests the important regulatory functions of these genes.

  • Reg 1α
  • GW112
  • Annexin-1
  • inflammatory bowel disease
  • representation difference analysis

  • Abbreviations used in this paper

    AP
    alkaline phosphatase
    GAPDH
    glyceraldehyde-3-phosphate dehydrogenase
    hr
    human recombinant
    IBD
    inflammatory bowel disease
    IL
    interleukin
    IFN-γ
    interferon γ
    TNF-α
    tumour necrosis factor α
    SIN-1
    3-morpholinosydnonimine
    PCR
    polymerase chain reaction
    DP
    difference product
    Dig
    digoxigenin
    ECL
    enterochromaffin
    fg
    femtogram
    RDA
    representation difference analysis
    Reg 1α R
    Reg 1α receptor
    UC
    ulcerative colitis
    ISH
    in situ hybridisation

    • https://doi.org/10.1136/gut.48.5.623

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    • Abbreviations used in this paper

      AP
      alkaline phosphatase
      GAPDH
      glyceraldehyde-3-phosphate dehydrogenase
      hr
      human recombinant
      IBD
      inflammatory bowel disease
      IL
      interleukin
      IFN-γ
      interferon γ
      TNF-α
      tumour necrosis factor α
      SIN-1
      3-morpholinosydnonimine
      PCR
      polymerase chain reaction
      DP
      difference product
      Dig
      digoxigenin
      ECL
      enterochromaffin
      fg
      femtogram
      RDA
      representation difference analysis
      Reg 1α R
      Reg 1α receptor
      UC
      ulcerative colitis
      ISH
      in situ hybridisation
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