Existing research on choice of online learning utilized factors such as perceived level of control, independence, and satisfaction, yet the relationship among these factors is under-researched. Due to the value of "learning anytime," biological factors underlying "choice of time" might provide additional insights. This article presents findings of a study with two aims: (1) investigating the relationship between previously described factors; and (2) researching the impact of a different theoretical framework, chronobiology, to provide insights in students' choices for online learning. Our research with traditional students taking online courses shows a statistically significant correlation between students' perceived independence, level of control, and satisfaction. Although there is no statistical significance between the three factors and the new factor of chronobiology, we found statistically significant results between students' chronotype and their choice of learning time for online classes. Implications and limitations are discussed. (Contains 4 tables and 2 figures.)