Student interest in artificial sweeteners can enhance the biochemistry classroom learning experience. This in class, guided-inquiry activity focuses on sucralose and fits into a 50-min biochemistry class for undergraduate science majors. Background knowledge of carbohydrate structure, function, and metabolism as well as familiarity with interpretation of primary literature is assumed. This activity uses short answer questions that stimulate small group discussion. (Contains 1 table, 4 figures and 3 notes.)