This investigation examines the relationship between prior disability-focused training and university staff members' attitudes toward students with learning disabilities (LD). A survey containing items pertaining to prior disability-focused training experiences and attitudes about students with LD was administered to 300 university staff members. Responses from 112 participants indicated that staff who had not received any form of prior training had greater interest in receiving training regarding LD and had insufficient knowledge to support students with LD. In contrast, staff who had received some form of prior training reported greater scores on general knowledge and sensitivity toward university students with LD. Analyses of training type indicated that staff who had previously participated in disability-related workshops and coursework reported the most positive attitudes, followed by staff who had participated in other forms of training (i.e., reading books and articles or visiting websites). Total number of training types experienced, time spent engaged in training activities, and the amount of time elapsed since training experiences predicted different aspects of staff members' attitudes and perceptions as well as their satisfaction with prior training. The implications of these findings for future disability-focused professional development efforts within postsecondary settings are discussed. (Contains 3 tables.)