In this laboratory exercise, students develop a LabVIEW-controlled high-performance liquid chromatography system utilizing a data acquisition device, two pumps, a detector, and fraction collector. The programming experience involves a variety of methods for interface communication, including serial control, analog-to-digital conversion, and instrument control via digital input-output. The assignment can be set up as a class project or students can be rotated through the different tasks as a part of the course. (Contains 1 figure.)