Gender differences in the construct validity of Jack's (1991) Silencing the Self Scale (STSS) were assessed using 187 female and 169 male undergraduates. Results of exploratory and confirmatory factor analyses revealed gender differences in the scale's latent structure and at best provided only limited support for the validity of the scale's four subscales for women. Among men, the studies exposed a weakness in the scale's construct validity and provided insights into possible alternative interpretations made by men when responding to the scale's items. Convergent and discriminant validity of the scale were assessed by correlating it with measures of neuroticism and attachment. Whereas the construct validity of the STSS for use with women was supported, evidence was weaker for men. For men, data suggest that the STSS, in part, taps a motive to avoid intimacy and relationship situations that limit one's sense of independence and autonomy, a construct incongruous with Jack's notion of self-silencing. (Contains 4 tables and 3 notes.)