In an effort to achieve high success in knowledge and technique acquisition as a whole, a biochemistry and molecular biology experiment was established for high-grade biotechnology specialty students after they had studied essential theory and received proper technique training. The experiment was based on cloning and expression of alkaline phosphomonoesterase (AP) from "Escherichia coli JM83." The strategies undertaken to deal with the failure of individual experiments and to ensure each experimental group get along well are discussed here. A reasonable evaluation system has been established to improve the effectiveness of teaching, which includes four items: self-evaluation, a group presentation, work on duty, and experimental reports. These items were monitored for contribution to the students' perceived cognitive achievement. Motivation for learning seems to have been improved in the laboratory, particularly by the involvement in a presentation of the experimental work and results. (Contains 4 figures and 4 tables.)