This study was designed to redevelop and examine the validity of the Rehabilitation Adherence Questionnaire (RAQ), which can be used in both research and medical rehabilitation settings. The participants in the study were composed of 240 injured athletes (first analysis 120, second analysis 120 athletes) who participated in a physical rehabilitation program. Scale development, descriptive analysis, and factor analysis (exploratory factor analysis, confirmatory factor analysis) developed 25 items of RAQ for injured athletes. RAQ had six subscales such as support from significant others (five items), pain tolerance (five items), scheduling (four items), self-motivation (five Items), perceived exertion (three items), and environmental conditions (three items). Criterion-related validity, differential validity, and test-retest reliability of RAQ indicated that RAQ can be used both in clinic-based study and rehabilitation settings. In summary, this study showed good psychometric properties of RAQ.