In 2004 UNAIDS and its development partners first introduced the Three-Ones Principles. Since then, many governments have developed national strategic frameworks and guiding policy documents to help coordinate more effective national responses in the battle to overcome AIDS. In this article, I outline how essential it is for government agencies, nongovernmental organizations, and development partners to build effective and synergistic network relationships to achieve a unified national response against AIDS. Several best practice country case study examples are provided along with specific practices that nations should avoid in an effective national HIV/AIDS response. The role of education is interwoven throughout each case country example with an emphasis on examining what works and what should be avoided in multisectoral development agency coordination efforts.