This study extends research on objectification theory as it applies to eating disorder symptomatology by examining whether its tenets generalize to women identifying as lesbian. Lesbian women (N = 181) and a comparison sample of heterosexual women (N = 196) completed measures of objectification theory constructs (interpersonal sexual objectification, body surveillance, body shame, interoceptive awareness, disordered eating). The model of objectification theory was tested separately for lesbian and heterosexual participants. Path analyses revealed that this model provided a poor fit to the data for the lesbian sample, whereas it provided an excellent fit to the data for the heterosexual sample. Based on the data from the lesbian participants, an exploratory model was evaluated. Overall, findings suggested that the interrelationships among the objectification theory constructs were different and more complex for the lesbian participants than for the heterosexual participants.