Video feedback is a commonly recommended tool for online physical education (OLPE) instructors. However, there is little evidence to support its effectiveness in motor skill learning. This study investigated the impact of different forms of pedagogical guidance delivered alongside video feedback on the acquisition and retention of two-disc golf skills: the spin putt (SP) and backhand throw (BT). Forty-three college students were randomly assigned to one of the three video feedback groups: visual cue sheet (VCS), self-assessment (SA), or delayed teacher feedback (TF). During four online practice sessions, they trained on the spin putt and backhand throw techniques. Pre-, post-, and retention tests assessed their performance on both skills. The results indicate significant improvements in BT technique at posttest for both SA and VCS groups. All participants exhibited significant gains in SP technique at posttest; however, only the VCS group sustained these improvements for both disc golf skills at the retention test. No significant differences were observed between the groups for both disc golf skills at all three measurements. These findings suggest that video feedback, particularly when combined with in-the-moment guidance like VCS or SA, can effectively enhance motor skill learning in online settings. This result underscores the potential of OLPE to go beyond fitness-based activities and effectively address psychomotor learning standards. Future research should explore the long-term impact of video feedback in OLPE and identify optimal guidance strategies for diverse motor skills and demographics.