Metabolism is always a challenging topic in biochemistry. In our project, a courseware called "Metabolism Metro" was developed. "Metabolism Metro" is a self-learning tool which aims to facilitate students learning of metabolic pathways in an interactive and easy-to-understand manner. The courseware includes both pre-class and post-class learning activities. There are three parts in this courseware: "Story Mode, Revision Mode," and "Adventure Mode." "Story Mode" contains animations and interactive exercises which act as pre-class learning aids for students to learn basic concepts of metabolic pathways. "Revision Mode" contains flashcards summarizing the functions of the biomolecules in metabolic pathways. "Adventure Mode" contains quizzes, which act as self-testing tools for students to gauge their understanding of the learning material presented in the courseware. The development of "Metabolism Metro" courseware was divided into six phases. The ADDIE (Analyze [right arrow] Design [right arrow] Develop [right arrow] Implement [right arrow] Evaluate) instructional design was adopted for the development of each phase of the courseware. At the end of the phase, feedback was collected from students for adjustment of the previous phase, and suggestions were also collected as reference for adding new features in the next phase. By dividing the development of "Metabolism Metro" courseware into smaller parts, we were able to adopt the ADDIE method in a more Agile way. This Agile approach allowed continuous improvement of the courseware, and thus, the sustainability of the project could be enhanced.