During their first year of study at university, many students encounter challenges in developing learning strategies that align with success in the courses in which they are enrolled. The emergence of the COVID-19 pandemic heightened the challenges as universities were compelled to transition to online learning. Therefore, this study investigated the self-reported use of learning strategies in a first-year chemistry course delivered online due to the COVID-19 pandemic to identify learning strategies associated with success. Grounded in self-regulated learning (SRL) theory, a case study approach with an explanatory mixed methods design was adopted. Quantitative data were collected using a hybrid of the Motivated Strategies for Learning Questionnaire and the Online Self-regulated Learning Questionnaire. Follow-up open-ended questions were emailed to the students for the qualitative part of the study. Statistical analysis of the quantitative data was performed using SPSS and RUMM2030, while thematic analysis was applied to the qualitative data. Students reported more frequent use of SRL strategies of environment structuring, effort regulation, and elaboration. Conversely, critical thinking, task strategies, help-seeking, and peer learning were reportedly used less often. SRL strategies linked with success in the course were identified as effort regulation, goal setting, and time management. The findings from the qualitative data revealed an impact of online learning due to the pandemic on the use of peer learning and help-seeking strategies. The paper discusses the implications of these findings for educational practices, particularly in the context of hybrid learning in the post-pandemic era.