The Autism Stigma and Knowledge Questionnaire (ASK-Q) was developed and validated to assess autism knowledge across cultural contexts. Given the wide international use of the measure, the current study aimed to re-examine the measurement properties of the ASK-Q. Using a large, international database (n = 5064), psychometric analyses examined both item discrimination and differential item functioning across four countries. These analyses identified 18 items in need of examination for removal or refinement and retention. Further review by a team of autism experts identified additional changes required to minimize item stigma and increase item cross-cultural sensitivity and accuracy. These changes resulted in the ASK-Q, Second edition (ASK-Q-2), a briefer measure, that maintained the original four-factor structure and excellent internal consistency, but that now has even higher reliability for use in cross-cultural contexts.