Several Nature of Science (NOS) studies have been conducted on different categories of professionals and different age groups. Even though NOS is considered a structural facet of scientific literacy, not much research has been conducted on the NOS ideas of scientists and science professionals. This crosssectional study investigated the nature of science (NOS) views of 40 participants who have or had exposure to chemistry research using a qualitative comparative analysis approach, in one chemistry department in a large Midwest R-1 university. The participants' chemistry research exposure varied from no research to 31 years of experience. Undergraduates, graduate students, postgraduate researchers, faculty, and scientists were recruited for this study to examine how their NOS understanding develops in relationship to their research exposure. These categories of participants demonstrated different and unique patterns of answers related to aspects of NOS. Science content knowledge and research exposure indeed enhanced science learners'/researchers' NOS understanding, but NOS understanding is not developed in direct proportion to the amount of science content knowledge or research experience that an individual gained. It was observed that professionals with the same expertise level responded to NOS questions similarly, and professionals with different levels of expertise answered NOS questions in significantly different ways.