This study assessed the psychometric properties of the Dutch translation of the Prosocial Behavior Questionnaire (PBQ-NL), a self-report questionnaire that distinguishes altruistic, reactive, and proactive prosocial behaviours. Participants were 381 Dutch adolescents and young adults aged 13-30 (M[subscript age] = 18.62, SD = 2.59, 65.1% female). Test-retest reliability data were available for 199 participants. A Confirmatory Factor Analyses supported the three-factor structure of the questionnaire, with adequate six-month test-retest reliability. Altruistic and reactive prosocial behaviours correlated significantly and positively with prosocial behaviours directed at family/peers and the larger society, but not with depression or aggression. Conversely, proactively motivated prosocial behaviour correlated significantly and negatively with prosocial behaviour directed at family and positively with experienced opportunities for contribution to the larger society, aggression, and depression. These findings show that the PBQ-NL is a reliable measure to disentangle self- and other-serving motivations that underly prosociality during adolescence and young adulthood.