We describe an iodometric titration method for the determination of ascorbic acid using a microfluidic paper-based analytical device([mu]PAD). The ascorbic acid was detected by measuring the length of the blue band formed in a straight paper channel delimited by wax. This analytical method was designed as a laboratory experiment for under graduates majoring in chemical education. The main objectives of this practice are to introduce [mu]PAD into a preservice teacher's classroom, motivating their passion of learning chemistry, and implementing green chemistry education. Finally, we hope this novel microscale chemistry experiment could be introduced into the chemistry classroom at high schools by these preservice teachers after graduation. A total of 145 preservice chemistry teachers performed this experiment in the fifth semester. They completed this experiment within 3 h; positive feedback from these students indicated that the learning objectives and instructional aims were met.