NotesFAQContact Us
Collection
Advanced
Search Tips
Back to results
Peer reviewed Peer reviewed
Direct linkDirect link
ERIC Number: EJ1443412
Record Type: Journal
Publication Date: 2023-Jun
Pages: 7
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
EISSN: EISSN-1938-1328
Simple Preparation Method of Silica Core-Shell Spheres for HPLC in Undergraduate Chemistry
Xiang Wan; Dian Wang; Yuqing He; Jiafei Wang; Qin Xu; Donglin Zhao; Fazhi Xie; Qishu Qu
Journal of Chemical Education, v100 n6 p2175-2181 2023
High-performance liquid chromatography (HPLC) experiments are an important part of the undergraduate analytical instrumentation laboratory courses and usually use commercial C18 silica-packed reversed-phase columns. In this experiment, a simple method of preparing SiO[subscript 2] core-shell packed columns was developed to enable students to master the whole process of HPLC experiment from stationary phase preparation and column packing to separation experiments, just as they do for gas chromatography. Nonporous SiO[subscript 2] particles with a particle size of approximately 2.2 [mu]m were prepared by a three-step dilution reaction using the sol-gel method. The product yield was 92.1%. Monodisperse and spherical SiO[subscript 2] core-shell particles (SiO2@dSiO[subscript 2]) with a particle size of about 2.4 [mu]m were prepared by a one-step hydrothermal method using the prepared SiO2 spheres as the core. The product yield was 83.3%. The prepared SiO[subscript 2]@dSiO[subscript 2] particles were derivatized with n-octadecyldimethylchlorosilane and then packed into a stainless steel chromatography column. A high column efficiency of 83,188 theoretical plates per meter was obtained for fluorene in reversed-phase mode for the separation of a mixture of polycyclic aromatic hydrocarbons. The column efficiency obtained with this method is the same as that of a commercial column packed with fully porous particles of the same particle size. The SiO[subscript 2] core-shell stationary phase preparation method developed in this experiment has the advantages of simple process, high yield, and good repeatability. The experiment can be designed to be completed over a period of 1-3 weeks. Inorganic material synthesis is another important aspect of this experiment.
Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A