ERIC Number: EJ1442806
Record Type: Journal
Publication Date: 2022-Oct
Pages: 7
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
EISSN: EISSN-1938-1328
Raman Scattering Spectroscopy on Strawberry DNA
Philip A. Kocheril; Makaela M. Jones; Jessica Z. Kubicek-Sutherland
Journal of Chemical Education, v99 n10 p3546-3552 2022
Raman scattering spectroscopies are important analytical tools in modern chemistry. Here, we describe the construction of the unamplified Raman scattering analysis (URSA) instrument: an inexpensive, open-table Raman spectrometer that uses a 532 nm diode-pumped laser as a light source. Upon application of this spectrometer to DNA extracted from strawberries with a soap solution and precipitated in isopropanol, two major Raman peaks with shifts of 740 and 2970 cm[superscript -1] were observed, consistent with previously published DNA Raman spectra. In this general article, we discuss the potential implementation of this work in an upper-level undergraduate laboratory, which would allow for hands-on exposure to both spectroscopic instrumental design and biochemical sample complexity.
Descriptors: Spectroscopy, Science Instruction, Chemistry, Lasers, Genetics, Plants (Botany), College Science, Undergraduate Study, Science Laboratories
Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: National Nuclear Security Administration (NNSA) (DOE)
Authoring Institution: N/A
Grant or Contract Numbers: 89233218CNA000001