This study aims to evaluate the laboratory safety perception, awareness and attitude of undergraduates toward the chemistry teaching laboratory by means of a self-administered questionnaire. The survey contains a sample size of 959 undergraduates in Tianjin University of Technology involving four schools. Instrument reliability and validity are also tested. The results show that most of the students have a more positive laboratory environmental perception and safety awareness. However, the laboratory safety attitude needs improvement. In addition, there is a significant difference for both gender and school. This result could lead to the improvement of the subsequent safety education.