ERIC Number: EJ1441710
Record Type: Journal
Publication Date: 2022-Dec
Pages: 8
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
EISSN: EISSN-1938-1328
Molecular Structure from Models to Mastery: An Inquiry-Based Study of Human Insulin-Degrading Enzyme
Michael A. Araujo; Alexandra A. Barrere; Selena-Rae Tirado; Candace E. Williams; Monica I. Strada; Benjamin J. Alper
Journal of Chemical Education, v99 n12 p4094-4101 2022
Using crystal structure data, site directed mutagenesis, and real-time kinetic assays, students designed, expressed, and purified engineered mutants of human insulin-degrading enzyme (IDE). Students designed their own IDE mutants following "in silico" analysis, used inverse PCR to generate mutant plasmid expression constructs, expressed mutant proteins in "E. coli", and tested their function "in vitro." Structural requirements for enzyme function were investigated. Students demonstrated a mastery of critical concepts in enzymology including principles of experimental design and evolutionary implications of enzyme structure-function relationships. Experimental methods for the analysis of macromolecular interactions and computational approaches to scientific inquiry were explored and applied. This investigation was conducted as part of the second-semester undergraduate biochemistry laboratory at Sacred Heart University.
Descriptors: Molecular Structure, Science Instruction, Science Laboratories, Science Experiments, Laboratory Experiments, Undergraduate Students, College Science, Biochemistry
Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A