In the past few decades, chemistry has evolved to interact with various disciplines to synergistically help tackle global challenges. This, in turn, requires that newer generations of chemistry students are trained to be more flexible in accepting and coordinating new concepts. In this experiment, pineapple was used as a key model to allow for the incorporation of multiple important concepts into the laboratory. Using carotenoid separation as a main goal, column chromatography, UV-vis spectroscopy, thin-layer chromatography, high-performance liquid chromatography, mass spectrometry, and principal component analysis can be included in a cohesive laboratory experiment. On the other hand, these activities were also designed to be modular, thus allowing instructors to add, remove, or modify the contents in a highly customizable manner. This makes it highly versatile and amenable to uncertain situations like unexpected university closure due to COVID-19related lockdown. Overall, this laboratory experiment serves as a practical example of how chemistry can help solve real-world problems while also allowing high flexibility in teaching management.