ERIC Number: EJ1437739
Record Type: Journal
Publication Date: 2024-Aug
Pages: 4
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-1935-7877
EISSN: EISSN-1935-7885
Climate Change and Plant Rhizosphere Microbiomes: An Experiential Course-Embedded Research Project
Journal of Microbiology & Biology Education, v25 n2 2024
The current and ongoing challenges brought on by climate change will require future scientists who have hands-on experience using advanced molecular techniques, can work with large data sets, and can make correlations between metadata and microbial diversity. A course-embedded research project can prepare students to answer complex research questions that might help plants adapt to climate change. The project described herein uses plants as a host to study the impact of climate change-induced drought on host-microbe interactions through next-generation DNA sequencing and analysis using a command-line program. Specifically, the project studies the impact of simulated drought on the rhizosphere microbiome of Fast Plants rapid cycling "Brassica rapa" using inexpensive greenhouse supplies and 16S rRNA V3/V4 Illumina sequencing. Data analysis is performed with the freely accessible Python-based microbiome bioinformatics platform QIIME 2.
Descriptors: Plants (Botany), Microbiology, Science Instruction, Teaching Methods, Genetics, Scientific Research, Correlation, Metadata, Simulation, Natural Disasters, Programming Languages, Information Systems, Climate, College Students, Laboratory Safety, Course Descriptions
American Society for Microbiology. 1752 N Street NW, Washington, DC 20036. Tel: 202-737-3600; e-mail: journals@asmusa.org; Web site: https://journals.asm.org/journal/jmbe
Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Identifiers - Location: Wisconsin (Madison)
Grant or Contract Numbers: N/A
Data File: URL: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1091725/