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ERIC Number: EJ1437739
Record Type: Journal
Publication Date: 2024-Aug
Pages: 4
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-1935-7877
EISSN: EISSN-1935-7885
Climate Change and Plant Rhizosphere Microbiomes: An Experiential Course-Embedded Research Project
James A. Parejko
Journal of Microbiology & Biology Education, v25 n2 2024
The current and ongoing challenges brought on by climate change will require future scientists who have hands-on experience using advanced molecular techniques, can work with large data sets, and can make correlations between metadata and microbial diversity. A course-embedded research project can prepare students to answer complex research questions that might help plants adapt to climate change. The project described herein uses plants as a host to study the impact of climate change-induced drought on host-microbe interactions through next-generation DNA sequencing and analysis using a command-line program. Specifically, the project studies the impact of simulated drought on the rhizosphere microbiome of Fast Plants rapid cycling "Brassica rapa" using inexpensive greenhouse supplies and 16S rRNA V3/V4 Illumina sequencing. Data analysis is performed with the freely accessible Python-based microbiome bioinformatics platform QIIME 2.
American Society for Microbiology. 1752 N Street NW, Washington, DC 20036. Tel: 202-737-3600; e-mail: journals@asmusa.org; Web site: https://journals.asm.org/journal/jmbe
Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Identifiers - Location: Wisconsin (Madison)
Grant or Contract Numbers: N/A