Heterosexism, the belief that heterosexual identities are superior to homosexual identities, is deeply embedded in educational institutions, including school-based agricultural education (SBAE). Negligible research exists developing impactful practices for queer students in SBAE programs. This study sought to describe the attitudes of post-secondary agricultural education students toward lesbian and gay identities to identify potential barriers to the inclusion of queer students in SBAE. A total of 119 students from 21 higher education institutions across 12 states completed the Multidimensional Scale of Attitudes Toward Lesbians and Gay Men. The questionnaire evaluated attitudes related to rejection of proximity, pathologizing of homosexuality, modern heterosexism, and support. Findings from this study reveal students are relatively supportive of lesbian and gay individuals, but do not challenge heterosexist beliefs. Recommendations include further qualitative and intersectional investigations, greater curricular inclusion of queer experiences, and providing opportunities to challenge homophobic and heterosexist group norms.