There are few measures of teacher professional knowledge of reading comprehension, therefore little is known about the levels of teacher professional knowledge, their motivation and attitudes to teaching reading comprehension. The Knowledge, Behaviour and Attitudes Test for Reading Comprehension (KBAT-RC) was developed from a systematic literature review, in collaboration with researchers and practitioners. Findings from the first sample suggest that teachers prefer more tangible practices when teaching reading comprehension (teaching vocabulary, prediction and asking questions), in contrast to developing the inference skills and self-monitoring for meaning strategies of the children they teach. Implications for the development of teacher professional knowledge in reading comprehension are discussed.