The article focuses on adaptation and validation of the Academic Motivation Scale questionnaire (AMS-28) in higher education in four Eastern European countries: Czechia, Slovakia, Serbia, and Poland. The research was conducted with a total of 1711 respondents. We examined the construct validity of AMS-28 including measurement invariance and reliability according to national, gender and age groups. Our analysis confirmed its original seven-factor structure as well as its reliability. The tool is measurement invariant across all compared groups (gender, age, countries) except Poland, whose results are specific. The results identify new places on the world map where AMS-28 is functional. The tool appears functional in time, space and various language mutations. Despite satisfactory results, there is still room for future examination of the AMS-28 among different countries. In practice, the tool has a wide range of application possibilities.