Science centres are informal learning spaces embedded with artefacts embodying mediational affordances. This exploratory and small-scale mixed methods study juxtaposes eye-tracking technologies and qualitative interviews to examine how visitors to a gallery navigated this space and interacted with different artefacts. A total of 15 visitors to the science centre gallery, Energy Story, participated in the study. The findings revealed inconclusive results about the directionality of their navigation. The mediational affordances of the artefacts, as interpreted from the interactive elements and interaction of the visitors and interviews, suggested that it was better to distribute the mediational affordances across a few artefacts in an exhibit rather than have one artefact embody several affordances. The concept of "mediational threshold" was suggested as a topic for future study. The findings contributed to the academic literature on eye-tracking studies at science centres. They also provided ideas for science centre curators and teachers who bring students with diverse learning needs to this mediational space.