Background: Traditional workplace interventions have targeted sedentary behavior using standing desk-based interventions delivered in the physical workplace. However, little is known about standing desk interventions designed for employees in flexible positions working at home and in the workplace. Purpose: The purpose of this study was to determine factors associated with the motivation to participate in and perceived benefits of a mobile standing desk intervention. Methods: After participating in an 8-week intervention using a mobile standing desk, employees (n = 20) completed one focus group session. Focus groups were audio recorded, transcribed verbatim, and analyzed using thematic analysis in NVivo. Results: Themes associated with motivation to participate included: 1) trialability; 2) previous experience; 3) health concerns; and 4) difficulty accessing a standing desk. Themes about perceived benefits of the intervention included: 1) awareness of workday behavior; 2) productivity; and 3) usefulness in the home setting. Discussion: Employees perceived work-related and health-related benefits from the mobile standing desk intervention. Motivating factors to use a mobile standing desk should be considered when recruiting employees for workplace interventions and programs. Translation to Health Education Practice: Findings from this study may help health education specialists design standing desk-based interventions for employees with remote and flexible work schedules. A AJHE Self-Study quiz is online for this article via the SHAPE America Online Institute (SAOI) http://portal.shapeamerica.org/trn-Webinars http://portal.shapeamerica.org/trn-Webinars