Background: Background: Research demonstrates the benefits of communities of practice (CoP) for teachers' professional learning in Physical Education (PE). However, much less is known about how CoPs can be used to understand pre-service teachers' (PSTs) professional learning in their Physical Education Teacher Education (PETE) programmes. Negotiation of meaning (NofM) is a crucial concept regarding learning inside of a CoP. Hence, understand how meaning is negotiated in initial teacher professional development might allow the enhancement of the social learning strategies in PETE programmes. Purpose: Purpose: This article draws on the CoP theoretical framework, and specifically the concept of NofM to understand how PSTs negotiated meaning during a two-year PETE programme. In particular, the focus is on the challenges faced by PSTs throughout the PETE social interactive process. Participants and settings: Participants and settings: The setting was a PETE programme in Portugal. In year one, the participants were three university teacher educators (TE) and 25 PSTs. In year two, the participants were three PSTs from year one who were in their school placement, a university supervisor (US) and one cooperating teacher (CT). Data collection and analysis: Data collection and analysis: This study was an ethnography with a two-year longitudinal design. Data were collected through the ethnographer's participant-observations (first author), meetings of the PSTs with the mentoring team (TEs, US, CT), and the ethnographer's field diary, reflexive log, and audio-recordings. Data analysis involved the constant-comparative grounded theory method. Findings: Findings: Analysis resulted in the identification of three key challenges during the course units, for PSTs in the first year of their PETE programme: (a) the diversity and disconnections within the course units; (b) (the lack of) membership; and (c) the absence of democratic spaces in PETE class discussions. In the year two of PETE during the school placement, three challenges were identified for the negotiation of meaning (NofM): (a) the lack of dialogue with and focus on pedagogical practice by the cooperating teacher; (b) working together and the pressure to individualise PSTs' education process; and (c) unreachable support and supervision from the university. Implications: Implications: We suggest that PETE programmes should emphasise pedagogical strategies that create spaces for the cultivation of CoPs. For instance, we suggest PST engagement in continuous and planned group work across curricular years (e.g. peer-teaching and micro-teaching experiences) to increase work group membership. We recommend the emphasis on discussion sessions mediated by a facilitator (e.g. TEs, CTs, or US) where the different working groups could share their repertoire of learning experiences as a CoP. Moreover, we suggest optimisation of a base of pedagogical knowledge regarding course units' integration between the TEs of the PETE to systematise the process and also, an increased connection between the university and the host school. Additionally, we propose continuous professional development by the CTs to facilitate the requirements of the PETE programme.