This study aimed at investigating the problems that translators faced when subtitling cultural expressions from English into Arabic. To achieve this goal, the researchers selected a convenient sample comprising 40 graduate and 40 undergraduate students who were enrolled in the English language programs during the academic year 2012/2013 in Jordanian universities. A translation test, which consisted of 10 cultural expressions selected from three American movies namely, "Scent of a Woman", "Erin Brockovich" and "Casino", served as the main instrument. Results revealed that translators encountered structural, lexical and semantic difficulties which are triggered by interference from L1 into L2. Also, results showed that translators lacked the ability to identify these cultural expressions due; perhaps, to the bilingual dictionaries they consulted which rendered meanings in isolation rather than in context.