Private school choice programs' success depends partly on the supply of private schools. Many parents prioritize a religious education for their children. Therefore, it is important to consider how religious private schools may respond to various program regulations. We conducted an experimental study surveying a national sample of 354 leaders of Association of Christian Schools International (ACSI) member schools, the second largest private school organization of any kind in the United States. We find evidence that while over four-fifths of Christian school leaders are inclined to participate with no changes to school operations, open enrollment mandates reduce participation rates by 62 percentage points and employment regulations reduce participation rates by 79 percentage points.